1. Gene synthesis using a bioinformatics tool and two steps PCR assembly.
2. Designing anticancer peptide using a bioinformatics tool.
3. Peptides in diagnosis and treatment.
4. Molecular diagnosis of HCV, HBV, and EBV using qualitative and quantitative PCR.
5. Molecular cloning of different genes (e.g. human interferon-gamma, HCV Core) into cloning and expression vectors.
6. Lab-scale and process development for limited pilot-scale production of recombinant human interferon-gamma and human interferon alfa 2b.
7. Batch and Fed-batch fermentation of genetically engineered E. coli strains.
8. Purification of recombinant therapeutic and diagnostic proteins using fast protein liquid chromatography (FPLC) and high-pressure liquid chromatography (HPLC).
9. Detection of cytotoxic activity of recombinant therapeutic proteins using mammalian cell cultures.
10. In vitro cytotoxicity and anti-inflammatory of natural and synthetic compounds.
