Enrichment analysis: interpret gene lists like a pro
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Enrichment analysis: interpret gene lists like a pro

How to get more information from the results of high throughput gene expression data with easy-to-use web tools?
3.3 (3 ratings)
Instead of using a simple lifetime average, Udemy calculates a course's star rating by considering a number of different factors such as the number of ratings, the age of ratings, and the likelihood of fraudulent ratings.
17 students enrolled
Last updated 8/2014
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  • 28 mins on-demand video
  • 8 Supplemental Resources
  • Full lifetime access
  • Access on mobile and TV
  • Certificate of Completion
What Will I Learn?
  • You will learn how to perform over-representation and gene set enrichment analysis on microarray and RNA-Seq
  • You will understand the relevant statistical approaches which are needed to find Gene Ontology, KEGG or other pathway terms which are associated with gene lists
  • You will be able to design and execute such analysis
  • You will be able to use GeneTrail and WebGestalt web tools
  • You will be able to interpret the results from such an analysis
View Curriculum
  • Basic knowledge of molecular biology
  • Very general idea about high throughput gene expression experiments (microarray, RNAseq)

Gene enrichment analysis is the most popular systematic approach to assign ontologies, pathways, and transcription factors to gene lists usually resulted from high throughput experiments. This short course introduces the two most frequently applied methods to locate the common features of large gene lists, and provide opportunities to practice this analysis in the most common research scenarios using GeneTrail (for gene set enrichment analysis) and WebGestalt (for over-representation analysis), the two web tools used most often in the field.

You get written material, video explanations for the background, and prepared example data files for practices. You are provided with (almost) real life discussions between a wet-lab biologist and a bioinformatician to clarify the most common misunderstandings related to this analysis. You are led step by step by two experts of the field, both active researchers; and in the meantime, you are challenged to proof your knowledge in quizzes.

If you have a solid background in molecular biology research, you will be able to expert these methods in a maximum of 8-10 active course hours, but if you have only a general idea of the field, you will get a plenty of background material to learn and practice all in a few days. We recommend to cover all the provided material approximately in a week to maximize the efficiency of your learning.

As you know, bionformatics and data analysis skills are highly demanded among molecular biology and pharma researchers. Why not equip yourself with these easy but very useful tools to extend your future potential as a researcher?

Who is the target audience?
  • Biotechnology university students
  • Molecular biology researchers
  • College students interested in bioinformatics
  • Clinical researchers
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Curriculum For This Course
Expand All 16 Lectures Collapse All 16 Lectures 43:26
Welcome to the Gene Enrichment Analysis course!
3 Lectures 01:44

Short guide to the course. What will you learn, and how can you use it?

Preview 01:44

Introduction and background.

Enrichment Analysis in General
2 pages

Definition of terms often used in context with enrichment analysis.

Related concepts
3 pages

Enrichment Quiz
6 questions
Over-Representation Analysis (ORA)
5 Lectures 10:58

Theory behind over-representation analysis.

Preview 01:37

What you need for analyzing over-representation and what you get as a result?

How to do an over-representation analysis?
2 pages

In this lecture, you have an opportunity to practice over-representation analysis with real life experimental data. If you have own data from your own research, please do not hesitate to test its performance. Otherwise, you can use the datasets from the supplementary material.

human_gene_set.txt – a list of 892 human genes identified by Entrez Gene IDs.

mouse_probe_set.txt – mouse genes identified by 247 probe IDs from Affymetrix Mouse430_2 platform.

Preview 04:14

Questions from the biologist and answers from the bioinformatician.

Preview 05:07

Most important concepts of over-representation analysis.

Summary for ORA
1 page

ORA Quiz
3 questions
Gene Set Emrichment Analysis (GSEA)
5 Lectures 14:32

Theoretical background of gene set enrichment analysis.

What is a gene set enrichment analysis?

What you need for gene set enrichment analysis, and how to understand the results.

How to analyse gene set enrichment?
2 pages

Step-by-step instruction to accomplish Gene Set Enrichment Analysis using a web service. You can use your own data or the set provided in the supplementary material.

mouse_ranked_gene_set.txt - a ranked list of 2300 mouse genes identified by Entrez Gene IDs.

Do your gene set enrichment analysis by yourself!

The biologist asks, the bioinformatician answers.

GSEA in a biologists eye

Most important concepts of gene set enrichment analysis.

Summary to GSEA
1 page

6 questions
Concluding remarks
3 Lectures 01:12

Comparison of ORA and GSEA.

Similarities and differences
2 pages

If you speak the language of computers...

R packages
2 pages

Short summary of the learned techniques.

Preview 01:12

4 questions
About the Instructor
Dr. Csaba Ortutay
3.3 Average rating
3 Reviews
17 Students
1 Course
Adjunct professor of bioinformatics

I have experience with bioinformatics related to human immunity, focused on databases, evolution and systems biology. I teach genetics, phylogenetics, and evolution at different Universities in Finland and Hungary.

My expert areas: computational and systems biology related to immune processes, molecular evolution of gene families.

I have conducted bioinformatics research during the past two decades using and developing algorithms and tools for answering research questions using computers as tools. I use (among many other methods) enrichment analysis routinely when I want to characterize larger gene or protein groups, and I have applied it for locating essential genes in human immune functions. I have published more than two dozen scientific publications from my results, for example, about disease gene identification, the evolution of immune functions, and biological networks.

Other areas I am involved: Biological databases, Genomics related web applications, University level teaching online and on-site.

PhD Zsuzsanna Ortutay
3.3 Average rating
3 Reviews
17 Students
1 Course
Molecular biologist, immunologist

My current project focuses on the role of proprotein convertase FURIN in T cell receptor signaling. My main fields of interest are the molecular mechanisms of T cell stimulation in health and diseases, and the role of glycans in immune mechanisms.

I investigated the role of glycosidase enzymes in the pathomechanism of rheumatoid diseases. I also have experiences with protein structure function studies.