In this course you will learn:
Polymerase chain reaction (PCR)
You will learn in this section:
Criteria for PCR primer design
You will learn in this section a detailed explanation of the PCR primer design criteria and how they affect the primer sensitivity and stability including; primer length, primer melting temperature, primer annealing temperature, GC% of the primer, GC-clamp, cross homology and primer secondary structure.
Tools and methods
In this section you will learn how to:
Gel Electrophoresis Troubleshooting
In this section you will find tips for successful gel electrophoresis. It includes all the possible problems you may encounter, and suggest you a solution for each problem.
This video provides an introduction to the Polymerase Chain Reaction (PCR) technique.
The video explains:
This video explains in details:
In PCR amplification, how many dsDNA products we get, where the two strands of the dsDNA, are with the same length of the target sequence?
Let's find out in this lecture!
Why we need to learn how to design a DNA primer?
Have you ever got a non-specific band in your gel electrophoresis runs? Or got a band with a different size than your target DNA? Well, I bet the answer is yes. How a primer sequence can lead to this?
In this session, we are going to learn how to design a DNA primer that really work.
First, let's watch this video that:
How does the primer length play a role in the designing criteria?
Well, let's find out in this lecture.
There is 4 approximation methods used to calculate the primer melting temperature (Tm), each one gives a reasonable result for a primer with a certain length.
In this lecture, we are going to explain in details, how to calculate the Tm using 3 calculation methods:
Keep your full attention.
Enthalpy, entropy, and free energy are somehow difficult for the beginners to understand instantly. This lecture explains them in a very easy way...
The nearest-neighbor method is more complicated than the other melting temperature calculation methods. In this lecture we simplified the complicated parts for you.
The Annealing temperature (Ta), is at which the primers anneal (or bind) to the single stranded DNA and form a hydrogen bond.
This lecture will explain:
This lecture explain
In this lecture, you will understand:
This lecture explains:
This lecture explains:
Learn how to measure the primer product length.
Study multiple cases that results in the low intensity of DNA bands
Study multiple cases that results in smeared DNA bands
I had a passion for biology from a young age as I used to focus on reading biology books more than anything else. At school time, I used to write biology assignments in a fun and creative way, drawing pictures and comics to transform the difficult parts of biology into easy and understandable pictures. I adore creativity, it is my lifestyle.