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Keep shoppingqPCR Setup for Success: Expert Training by QPCR Academy
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qPCR Setup for Success: Expert Training by QPCR Academy
A practical guide to the key considerations for planning and setting up reliable, reproducible qPCR experiments.
Created byKasia Ewert-Krzemieniewska, PhD
Last updated 8/2026
English
What you'll learn
- Understand the nine essential steps of a reliable qPCR workflow - from assay design and sample preparation to qPCR run setup.
- Identify the key parameters to consider when designing qPCR primers and probes for reliable assay performance.
- Plan sample collection, storage, nucleic acid preparation, and quality assessment to preserve sample integrity.
- Set up reverse transcription correctly and select suitable reagents and laboratory plastics.
- Choose and apply appropriate qPCR controls, replicates, and contamination-prevention practices.
- Set up a valid qPCR thermal cycling protocol for the selected assay and detection chemistry.
- Identify and prevent common workflow mistakes that can compromise qPCR accuracy and reproducibility.
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Course content
10 sections • 10 lectures • 1h 2m total length
Requirements
- You already understand the basic principles of PCR and qPCR but lack hands-on confidence and need practical guidance when setting up your own experiments.
Description
This course contains the use of artificial intelligence.
You learn PCR at university.
You revisit it during laboratory classes.
You may even perform it as part of a practical course or your first research project.
And yet, when it is time to plan and set up your own qPCR experiment, you may still be unsure where to begin. With so many factors to consider - from assay design and sample preparation to controls, reagents, plate setup, and cycling conditions - it can be difficult to know whether you have set everything up correctly.
qPCR is a core technique in molecular biology, diagnostics, and biotechnology. However, many students and laboratory professionals never receive structured, practical training covering the complete experimental setup workflow.
This course is designed for learners who already understand the basic principles of PCR and qPCR but need practical guidance and greater confidence when planning and setting up their own experiments.
In less than two hours, you’ll work through the complete qPCR experimental setup workflow- all in one place. You’ll learn how to plan each step, avoid common setup mistakes, and create the right conditions for accurate and reproducible results.
Discover nine workflow steps you can immediately apply to plan and set up qPCR experiments more confidently in your own laboratory.
Who this course is for:
- Students and PhD researchers who are learning qPCR or want to strengthen their practical skills
- Scientists and laboratory professionals working in academia, biotechnology, pharmaceuticals, diagnostics, food testing, wastewater surveillance, forensics, or animal health who use or plan to use qPCR.
- Anyone experiencing inconsistent qPCR results who wants to identify and prevent common experimental setup errors.