Molecular Biology basics: Unlocking Life's Code!

Introduction To DNA

Introduction to the course

DNA is the genetic material Experiments

• Griffith’s Transformation Experiment (1928).

• Avery, MacLeod and McCarty’s Experiment (1944).

• Hershey and Chase’s Experiment (1952).

DNA structure and function

• DNA structure.

• DNA role and function.

DNA organization inside the cell.

• DNA organization from double helix to chromosomes.

DNA Epigenetic Mechanisms of Gene Expression

DNA Methylation

• DNA methylation mechanism definition.

• DNA methylation mechanism role.

Nucleosome remodeling

• Nucleosome remodeling mechanism definition.

• Nucleosome remodeling mechanism role.

Histone modifications

• Histone modifications mechanism definition.

• Histone modifications mechanism role.

Non-coding RNAs

• Non-coding RNAs mechanism definition.

• Non-coding RNAs mechanism role.

DNA replication

The central dogma

• The central dogma definition.

• DNA replication introduction.

Origin of replication

• Origin of replication process in eukaryotes.

DNA Replication Enzymes - Part 1

• DNA helicase.

• Single-strand binding protein.

DNA Replication Enzymes - Part 2

• RNA primase.

• DNA polymerase I, II, and III.

DNA Replication Enzymes - Part 3

• DNA ligase.

• Topoisomerase.

Synthesizing a New DNA Strands

• Synthesizing the leading strand.

• Synthesizing the lagging strand.

DNA damage repair

DNA Damage Repair Mechanisms - Part 1

• Mismatch repair mechanism.

• Base excision repair mechanism .

DNA Damage Repair Mechanisms - Part 2

• Homologous recombination & Non homologous recombination mechanisms.

• Nucleotide excision repair mechanism.

• Direct damage reversal mechanism.

DNA Transcription

DNA Transcription Process and Steps

• DNA transcription.

• Initiation stage.

• Elongation stage.

• Termination stage.

Post-Transcriptional Modifications

RNA Processing

• RNA processing mechanism.

Initiation of Translation and mRNA Degradation

• initiation of translation and mRNA degradation mechanisms.

Protein Processing and Degradation

• Protein processing and degradation mechanisms.

DNA Translation

DNA Translation Process and Steps

• DNA Translation.

• Initiation stage.

• Elongation stage.

• Termination stage.

Post-Translational Modifications

Protein Folding and Targeting Polypeptides to Specific Locations

• Protein Folding.

• Targeting polypeptides to Specific Locations.

Gene regulation

• Gene regulation control.

• Transcription factors.

• Enhancers.

• The TATA box.

• Exons and introns