Molecular Biology and biotechnology principles

In this course students will learn about molecular biology and biotechnology principles.

1.  Unit 1

Topics covered in : Lecture 1 , 2 and 3

• Recombinant DNA technology

• Tools used in Recombinant DNA technology

• Isolation of vector gene

• Isolation of desired gene

• Methods to transfer foreign gene into bacterial cell

• Discuss about Restriction enzymes

• And about ligases

• Vectors like cosmid, plasmid

• Production of insulin gene

• Applications of recombinant DNA technology

  
  

2. Unit 2

Topics covered in Lecture 4

Molecular biology Techniques

Southern blotting technique used for DNA samples

Northern blotting used for RNA samples 

3. Unit 3

Topics covered in Lecture 5

• Hybridisation technique

• Polymerase chain reaction used to amplify the DNA in the sample

• Radio immuno Assay - Antigen and antibody specific

• Electrophoresis

• zone electrophoresis

• Gel electrophoresis

• Polyacrylamide gel electrophoresis.

• Paper electrophoresis.

• Principle of electrophoresis.

• Used to separate the DNA depending on the size and charge on the molecule.

• Gel electrophoresis - is used to separate DNA or RNA.

• SDS page used to separate proteins and amino acids

  
  

4. Unit 4

Topics covered in Lecture 6 and 7

• chromatography

• column chromatography

• Affinity chromatography.- based on affinity towards ligand and protein .

• Ion exchange chromatography.

• Cat-ionic exchanger and anionic exchanger

• Separation is based on ions present in the protein sample

• Gas liquid chromatography. Gas acts as a mobile phase and liquid acts as a stationary phase .gases like inert gases are used and liquid of high boiling point is used in gas liquid chromatography

• Paper chromatography . Paper is used and reagent are used to identify the compound present in the sample

• principle of chromatography

• separation of molecules based on size, charge and molecular weight

• Separation of macro molecules based on antigen and antibody interaction

• Enzyme and ligand interaction as there is a specific interaction between the antigen and antibody interaction

    Summary of the course

Add-on

• Course would be added/enhanced based on Q&A form students in to form of additional information