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Light and Transmission Electron Microscope Basics
Rating: 4.0 out of 5(4 ratings)
483 students

Light and Transmission Electron Microscope Basics

Foundations of Imaging and Microstructural Analysis
Created byAtasi Ghosh
Last updated 7/2025
English
English [Auto],

What you'll learn

  • Understand the Fundamental Principles of Microscopy
  • Scope of Light and Electron Microscopy Techniques
  • Develop Practical Skills in Sample Preparation and Imaging
  • Interpret Microstructural Features from Microscopic Images

Explore related topics

Course content

2 sections • 17 lectures • 1h 59m total length

  • Principle of Image formation10:10

    Explore optical principles of light microscopes, including resolution, magnification, and depth of focus, and the infinity corrected design with objective, tube lens, and eyepiece.

  • Factors affecting resolution9:11

    Explore how brightness, contrast, and depth of field govern resolution in light and transmission microscopes, influenced by numerical aperture, magnification, and lens aberrations.

  • Illumination system2:04

    Explore the optical microscope illumination system, including the Keeler setup, condenser lens, aperture and field planes, diaphragms, and filters, then examine objective and eyepiece aberration markings and micrometer use.

  • Imaging modes6:23

    Explore imaging modes of a light microscope, including bright field, dark field, phase contrast, polarization microscopy, and Nomarski differential interference contrast, highlighting anisotropy, birefringence, and three-dimensional relief.

  • 3D Confocal Microscopy0:56

    Explore three-dimensional confocal microscopy and its optical resolution for biological samples, using a laser light beam, scanning device, and pinhole aperture to build a three-dimensional image revealed by fluorescent dye.

Requirements

  • No prerequites

Description

This course provides a foundational understanding of light microscopy and transmission electron microscopy (TEM), two essential techniques for imaging and analyzing microstructures in materials and biological specimens. Designed for students and early-career researchers, the course introduces the operating principles, instrumentation, and applications of both light and electron microscopy. The first part of the course focuses on light microscopy, covering concepts such as magnification, resolution, contrast mechanisms (bright field, dark field, and phase contrast), and sample preparation methods. Emphasis is placed on optical limitations, imaging artifacts, and practical usage in routine lab analysis. The second part introduces transmission electron microscopy, delving into electron-matter interactions, beam generation, electromagnetic lenses, and image formation. Students will learn how to interpret TEM images, understand diffraction patterns, and appreciate the high-resolution capabilities of electron microscopy. By the end of the course, students will be equipped with the fundamental knowledge and practical awareness required to select appropriate microscopy techniques, prepare samples effectively, and critically interpret microscopic images for research and development purposes. This intensive course introduces the core principles and practical aspects of light and transmission electron microscopy (TEM), focusing on their applications in materials characterization. Designed for professionals, researchers, and graduate students, the course offers a concise overview of imaging fundamentals, resolution limits, contrast mechanisms, and sample preparation techniques. Participants will learn how to operate optical microscopes for routine inspection and understand the capabilities of TEM for high-resolution analysis of microstructures, interfaces, and defects. Key topics include diffraction patterns, beam-sample interactions, and contrast interpretation. Emphasis is placed on selecting the right technique for specific applications and recognizing imaging artifacts.

Who this course is for:

  • Beginner Materials Engineer, Mechanical engineers

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