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Mini-course on SDS-PAGE
Rating: 2.9 out of 5(4 ratings)
238 students

Mini-course on SDS-PAGE

The essentials of SDS-PAGE technique gel preparation, protein separation, and staining in under an hour.
Created byOmnia Ali
Last updated 1/2022
English
English [Auto],

What you'll learn

  • sodium dodecyl sulphate–polyacrylamide gel electrophoresis technique

Course content

1 section • 8 lectures • 31m total length

  • Introduction3:27

    Explore the SDS-PAGE technique for separating proteins by molecular weight using acrylamide gels, including the history of electrophoresis, the stacking effect, and modern methods.

  • properties of SDS-PAGE4:20

    Explain how SDS-PAGE separates proteins by size using an acrylamide matrix and SDS to mask charge, enabling denaturation and uniform migration for accurate molecular weight estimation.

  • Gel production5:53

    Learn to produce SDS-PAGE gels by casting acrylamide between two glass plates with spacers, using radical initiation to form stacking and separating gels at 3–4% and 10–12%.

  • Sample preparation2:36

    Prepare samples with the sample buffer, heat to denature proteins, reduce disulfide bonds with DTT, and load samples with a protein marker for gel-based size estimation.

  • Electrophoresis4:48

    Explore electrophoresis and how proteins separate by size in a buffer under applied voltage, using a size marker to reveal smaller proteins migrating faster and forming distinct bands.

  • Gel staining5:54

    Visualize proteins in SDS-PAGE with gel staining, using a blue stain and size marker to estimate sizes. Relate color intensity to protein quantity, with Western blot as a complementary method.

  • Applications2:27

    Explains applications of SDS-PAGE to separate and analyze proteins, enabling molecular mass determination and protein identification via Western blot, including diagnostic uses like HIV proteinuria testing.

  • variants and alternatives2:10

    Explore variants and alternatives in SDS-PAGE workflows, including native chromatography, enzyme activity detection, and precipitation methods such as ammonium sulfate and polyethylene glycol.

Requirements

  • no requirements

Description

This Mini-Course on SDS-PAGE is your quick-start guide to mastering Sodium Dodecyl Sulphate–Polyacrylamide Gel Electrophoresis (SDS-PAGE)—a core technique used for separating proteins based on size. Commonly applied in biochemistry, molecular biology, immunology, and pharmaceutical research, SDS-PAGE is essential for anyone working with protein samples.

Whether you’re a student learning lab techniques for the first time, a researcher refreshing your skills, or a technician supporting experimental workflows, this course delivers everything you need to know in a concise and practical format.

You’ll begin with a clear introduction to the principles and history of SDS-PAGE, followed by a step-by-step guide to gel and sample preparation, electrophoresis setup, and gel staining. Visual demonstrations help you understand each stage of the process, along with valuable tips for optimizing your results.

We'll also explore applications of SDS-PAGE, common issues and troubleshooting strategies, and introduce related techniques like Western blotting for further protein analysis.

Designed to be short but content-rich, this course helps you gain practical lab knowledge without spending hours on theory. The goal is to get you ready to perform SDS-PAGE confidently in any laboratory setting, following correct scientific procedures.

By the end of this course, you’ll have a strong understanding of how SDS-PAGE works and how to apply it effectively in your experiments. If you're looking for a focused, easy-to-follow, and scientifically accurate course, you're in the right place.

Who this course is for:

  • scientists and biological students

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