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Learn HPLC samples preparations and calculations
Rating: 5.0 out of 5(2 ratings)
7 students

Learn HPLC samples preparations and calculations

HPLC, Quality control, Sample preparation, Chemical laboratory
Created byAmr Tarek
Last updated 2/2025
Arabic

What you'll learn

  • Preparation of mobile phase
  • Preparation of standard and sample
  • Calculation for assay
  • How to identify the peaks using relative retention time
  • Preparation of solutions for related substances (standard & test)
  • Injection sequence for related substances solutions
  • Detection of related substances peaks
  • Types of calculations of related substances test
  • Calculations of related substances test
  • Related substances calculations equations
  • Precautions for related substances test

Course content

1 section • 13 lectures • 2h 25m total length
  • Introduction2:29

    Introduction to the course

  • Preparation of mobile phase23:04
  • Preparation of standard and sample for assay26:07
  • Calculations for assay19:15
  • Example on assay calculation1:15
  • Preparation of solutions for related substances7:00
  • Injection sequence for related substances solutions6:52
  • Detection of related substances peaks11:12
  • Types of calculations for related substances test5:54
  • How to identify the peaks using relative retention time7:33
  • Calculations of related substances test7:32
  • Related substances calculations equations22:26
  • Precautions for related substances test4:36

Requirements

  • At least 6 months experience is needed to understand this course

Description

Welcome to every one who is interested in pharmaceutical quality control field and thanks for your participation in this course.

This course is an advanced course for HPLC samples preparation and calculations, It is highly recommended to quality control analysts with minimum six years experience in quality control laboratory.

In this course, I will present thirteen lectures.

You will learn details about mobile phase preparation (Buffer solution preparation, pH adjustment, loading the isocratic and gradient injection mode, creating a sequence for assay solutions preparations).

Samples preparation for assay test (test solution and standard solution).

How to calculate the assay result using the suitable equation with an example.

You will know what is the related substances.

You will learn how to prepare all solutions needed for related substances test.

You will learn types of related substances calculations.

You will know the equation of each type.

You will see examples for each equation calculation.

You will know all the critical precautions that you must follow upon preparing related substances solutions in order to avoid any interfering error.

At the end of this course you will be able to deal with any pharmacopeia monograph and understand all tips and tricks, You will be able to deal with all (standards, samples and reagents) practically without any obstructions.

Finally, I would be appreciate to evaluate this course after participating and don't hesitate to contact me if you want to understand any information in this course.

Who this course is for:

  • All QC analysts with at least 6 months experience