Learn DNA Primer Design for Polymerase Chain Reaction
What you'll learn
- Learn how to design DNA Primer for any PCR Test like SARS-nCoV2, AIDS Detection
- Understand Mapping and Sequencing of genomes, Cloning, Basic Research
- Understand Basic feature Polymerase Chain Reaction and their Steps
- Learn two Bioinformatics tools used for manual method primer designing Cluster W Oligucalculator
- Learn one Bioinformatics tools used for manual method primer designing Primer 3
- Understand specific Parameters of Primer Design
- Used Forensics Science filed for DNA Amplification
- Basic Knowledge Computer
- Basic Science Knowledge
In this Bioinformatics course you will be find out how to DNA Primer Design for polymerase chain reaction. Primer BLAST performs only a specificity check when a target template and both primers are provided. Design primers for single- or multi-insert cloning or for your site-directed mutagenesis experiment (insertion, deletion, replacement) with our primer design tool. Primer3 is a computer program that suggests PCR primers for a variety of applications, for example to create STSs (sequence tagged sites) for radiation hybrid mapping, or to amplify sequences for single nucleotide polymor- phism discovery
Polymerase chain reaction (PCR) steps
Specification of Primer Design
Aim for the GC content to be between 40 and 60% with the 3' of a primer ending in G or C to promote binding
A good length for PCR primers is generally around 18-30 bases.
Try to make the melting temperature (Tm) of the primers between 65°C and 75°C, and within 5°C of each other
A good length for PCR primers is generally around 18-30 bases. Specificity usually is dependent on length and annealing temperature. The shorter the primers are, the more efficiently they will bind or anneal to the target.
However, a primer should not be too long 30-mer primers) or too short. Short primers produce inaccurate, nonspecific DNA amplification product, and long primers result in a slower hybridizing rate. ... One also needs to avoid primer-primer annealing which creates primer dimers and disrupts the amplification process
Who this course is for:
- Undergraduate Student
- Master Student
- Entry Level Primer Designer
- Biotechnology and Bioinformatics
Graduate in Biotechnology and Bioinformatics
I am working with search groups filed by various Bioinformatics. I am writing an article on Bioinformatics such as an informed document. Research interests involve genomic information, genomics and Bioinformatics.
Currently I have write some Prediction of Olea Europaea Compounds as an Inhibitor of Main-Peptidase
of SARS-nCoV2 using Molecular Operating Environment.