
Master an rna-seq data analysis workflow—from library construction and sequencing to quality control, mapping to a reference genome, de novo assembly, and differential expression with gene ontology and kegg pathway.
Explore RNA-seq data analysis with iDEP, from CSV upload and pre-processing to PCA, DEG analysis, and genome visualization, including heat maps, GO/KEGG pathways, and WGCNA networks.
Learn to run RNA-seq expression analysis in iDEP using counts or FPKM, preprocess data, identify degs, and explore heat maps, PCA, enrichment trees, and pathways.
learn to analyze public rna-seq datasets using the edap tool, prepare expression and design files, upload data, pre-process, and interpret differential expression results.
Use the Shining Go online tool to perform gene ontology enrichment analysis, build enrichment charts and kegg pathways, and analyze protein-protein interaction networks for genome-wide or proteome data.
Learn WGCNA via an online tool with no coding, using Arabidopsis thaliana gene counts and CSV design file to form modules, identify hub genes, and compare RPM and Mgcl2 treatments.
Learn how to create and interpret RNA-Seq data using Veni 2.1 Venn diagrams, upload gene lists, and identify common and unique genes across control and treatments.
Explore how principal component analysis interprets RNA-Seq gene expression, revealing treatment correlations, variation, and outliers through PC1 and PC2 in score and scree plots.
Learn how to perform principal component analysis using the SR plot website, including data formatting, sample and group design, and exporting PCA visuals.
Explore how to interpret gene ontology term analysis, distinguishing biological processes, cellular components, and molecular functions, and learn to report enriched GO terms for differential expression results.
Perform gene ontology enrichment analysis using cluster profiler and path view on the sr plot site, with two-column input of gene IDs and logfc.
Explore how to create a KEGG enrichment pathway graph using the SR Plot web tool, input enrichment factor, p value, and gene counts to generate publication-ready figures.
Interpret KEGG pathway analysis using bubble charts, focusing on rich factor, p value, and bubble size to select a representative pathway—a bubble with richness near one and low p value.
Explore how to create circular gene ontology visuals using the ggplot-based web tool, upload Excel data, customize layouts and colors, and export high-quality figures.
Create phylogenetic trees and sequence alignments using Atoll and Mega file formats. Export to Newick, and customize layout, colors, branch lengths, and labels for manuscript submission.
Learn to create colorful phylogenetic trees with a free ggplot web tool, upload Newick data, customize layouts and colors, add metadata, and export PNG visuals.
Learn to interpret a phylogenetic tree by identifying taxon tips, branches, and nodes, understanding clades, sister taxa, branch lengths, and bootstrap values.
Learn to create an SNP density plot with the SRW plot website by preparing a three-column file (SNP name, chromosome, position) for Brassica napus, then export a PDF.
Learn to draw a Circos plot using a web-based tool with two tracks (outer and inner), input data from a spreadsheet, and export PNG or SVG for genomics research.
Design a gene ontology plot using the SR plot web tool by inputting gene IDs, GO term IDs, and logfc values, then customize order and colors and export the PNG.
Learn to draw alluvial plots using the SRW plot web tool without coding, by preparing a three-column data set (circular RNAs, micro RNAs, messenger RNAs) and exporting PDF, PNG, SVG.
Create a heat map with SR plot online tool, input transcriptomic data for wild type and mutant type replicates, customize colors and clustering, and export PNG, SVG, TIF, or PDF.
Are you a student or researcher in biology, biotechnology, agriculture, medicine, or life sciences who struggles with data analysis due to limited coding experience? You're not alone—and you don’t need to become a data scientist to publish quality research.
I’m Dr. Asif Ali, a molecular biologist, educator, and the creator of Dr. Asif’s Molecular Biology. After completing my PhD and two postdoctoral fellowships in genomics and biotechnology, I spent years learning tools like Linux, R, and Python. Eventually, I realized that many researchers simply need a faster, simpler way to analyze and visualize their data—without getting overwhelmed by programming.
That’s why I created this course. It’s the shortcut I wish I had when I started. This course is specifically designed for students and researchers who want to generate professional-quality bioinformatics results using only web-based platforms—no software installation, no command lines, and no prior coding required.
Throughout this course, you'll learn how to use more than 20 freely available online tools to perform real-world biological data analysis. You'll be able to create high-quality heatmaps, volcano plots, bar graphs, boxplots, and other visualizations essential for your research. We'll walk through key workflows like RNA-seq analysis, WGCNA, GWAS, phylogenetic trees, and gene expression studies—all using intuitive, drag-and-drop interfaces.
You’ll also gain the confidence to interpret your outputs correctly for your thesis or manuscript. By the end, you'll be equipped to craft clean, scientifically sound figures for publications, scholarship applications, or grant proposals—without spending months learning R, Python, or Linux environments.
This course is ideal for students and researchers from biology-related fields who need practical results without programming. It is also helpful for professors seeking simple tools to enhance their teaching, and for anyone who wants to carry out complex biological analyses in a user-friendly way.
If you’re ready to save time, reduce frustration, and take your research presentation to the next level—this course is for you.