Introduction to Bacteriology

Name: Introduction to Bacteriology
Rating: 3.9 (14 reviews)

Staining Techniques, Culture Media and Methods, Biochemical tests, Infection and their sources. Microbiology, Bacteria.

Created byNithya Sudhakar

Last updated 10/2020

English

What you'll learn

What are Microorganisms?
What are prokaryotes and eukaryotes?
What is simple, negative and differential staining?
Procedure for Gram staining and Acid Fast staining
Shapes of Bacteria
Arrangement of Cocci, Bacilli
Why do we culture?
Solid, Liquid and Semi-solid media
What is agar?
Different types of Media
Simple or Basal Media
Complex Media
Special Media
Types of Culturing techniques used in laboratories
Fermentation and Biochemical properties
What is infection?
Classifications of Infection
What are the sources of infection?
What are biological and mechanical vectors?
Methods of Transmission of infection

Course content

5 sections • 16 lectures • 1h 7m total length

Introduction to the course1:49
Explore fundamental bacteriology topics, including microorganisms, prokaryotes and eukaryotes, sizes, microscopes, and staining. Identify shapes of bacteria, culture media, culturing, fermentation, biochemical properties, and infection transmission; includes end-of-section Q&As.
What are microorganisms, prokaryotes and eukaryotes5:50
Explore microorganisms and the distinction between prokaryotes and eukaryotes, detailing structures like nucleoid and nucleus, cell wall, capsule, plasmid, cytoplasm, ribosomes, and flagella.
Size of Bacteria and Types of Microscopes3:21
Discover the size range of bacteria from 0.2 to 1.5 micrometers in diameter and 3 to 5 micrometers in length. Learn how optical, phase-contrast, dark-field, and electron microscopes visualize bacteria.
Staining Techniques6:15
Explore staining techniques to visualize bacteria and differentiate live from dead cells, using simple, negative, and differential stains like Gram and acid-fast staining.
MCQ

Introduction to Section 20:53
Explore the shapes of bacteria and the purpose of culture media, including solid, liquid, and semi-solid media, agar, and the variety of simple, basal, and special media used in microbiology.
Shapes of Bacteria3:37
Explore the shapes of bacteria—cocci, bacilli, vibrios, spirilla, spirochetes, filamentous, and mycoplasmas—and how cell-wall defects form protoplasts and L forms; map arrangements like diplococci, streptococci, tetrads, and staphylococci.
Culture Media - Part 15:36
Explore culture media in bacteriology, from liquid broths to solid agar, learn how colonies on solid media aid identification, and survey aerobic, anaerobic, enriched, and selective media.
Culture Media - Part 26:41
Explore culture media types from simple to enriched, selective, differential, and indicator media, with examples like nutrient broth, blood agar, Mac Conkey agar, and Stuart's medium.
MCQ

Introduction to Section 30:45
Explore culture methods, learn why we culture bacteria, study each culture technique in detail, and identify bacteria through fermentation, biochemical properties, and laboratory tests.
Culture Methods4:28
Explore culture methods to isolate bacteria, demonstrate properties, and assess antibiotic sensitivity using streak, lawn, stab, pour plate, sweep plate, and liquid cultures to maintain stock cultures.
Identification of Bacteria 17:46
Identify bacteria from pure cultures by morphology, staining, and fermentation and biochemical properties, including IMViC tests and sugar fermentation, indole, MR, VP, citrate, and nitrate reduction.
Identification of Bacteria 24:39
Master catalase and oxidase tests to differentiate staphylococci from streptococci, pseudomonas and neisseria, and apply egg yolk, potassium cyanide, and tsi for fermentation and hydrogen sulfide in gram negative bacilli.
MCQ

Introduction to Section 40:24
Explore what infection is and identify the sources and methods of transmission of infection in section four of the introduction to bacteriology course.
Infection5:26
Explore host–microorganism interactions that define infection and infectious disease, including saprophytes, parasites, pathogens, and commensals, classify infections as primary, reinfection, secondary, focal, nosocomial, iatrogenic, endogenous, exogenous, subclinical, atypical, latent.
Sources of Transmission5:05
Explores the sources of infection, detailing humans as carriers (healthy, convalescent, temporary, chronic, contact, paradoxical), animal reservoirs and zoonoses, vectors and mechanical vectors, soil and water reservoirs, and contaminated food.
Methods of Transmission of Infection5:21
Explore the methods of transmission of infection, including direct and indirect contact, inhalation, ingestion, inoculation, vectors from insects, congenital (vertical) transmission, iatrogenic, and laboratory infections, with representative examples.
MCQ

Requirements

Basic biology in high school
A basic computer and internet connection

Description

You will learn about staining techniques, shapes of bacteria, arrangement, culture media, culture methods, biochemical tests, various types of infection, their sources, and methods of transmission. The last section contains 50 questions which will help you thoroughly understand the subject.

Being my first course, I have tried to keep it simple.

Who this course is for:

Graduates in Microbiology, Biochemistry, Medical and Applied Microbiology, Bacteriology

Introduction to Bacteriology

What you'll learn

Explore related topics

Course content

Section 14 lectures • 17min

Section 24 lectures • 17min

Section 34 lectures • 18min

Section 44 lectures • 16min

Section 50

Requirements

Description

Who this course is for: