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HPTLC Technique
Rating: 4.4 out of 5(53 ratings)
1,476 students

HPTLC Technique

HPTLC
Created byAnchrom Lab
Last updated 10/2021
English
English [Auto],

What you'll learn

  • Learn basics of HPTLC
  • Principle of TLC and HPTLC
  • You will learn about all the current HPTLC instrumentation available in market
  • Course will ensure thorough knowledge of HPTLC

Course content

1 section • 5 lectures • 1h 54m total length

  • Introduction26:54

    This introduction to hptlc explains the evolution from thin layer chromatography, outlines the three phases (sample application, development, and the third phase), and highlights automation and reproducibility.

  • Introduction on HPTLC - Part B16:27

    Explore high performance thin layer chromatography hptlc fundamentals, including post-chromatographic derivatization, visualization by spray or immersion, scanning and documentation of chromatograms, and method development and validation for diverse applications.

  • HPTLC Applications - Part A20:03

    Explore high-performance thin-layer chromatography applications across pharmaceutical quality control, identification, bioavailability, and impurity analysis, including plant extracts, food, cosmetics, and forensic uses.

  • HPTLC Applications - Part B18:43

    Explore high performance thin layer chromatography (HPTLC) applications across food and cosmetics, detecting adulterants, banned additives, and quality markers to ensure product authenticity and safety.

  • HPTLC Hyphenation and Effect Directed Analysis32:48

    Explore the principles of HPTLC hyphenation with mass spectrometry, enabling rapid separation, identification, and effect directed analysis across pharmaceuticals, herbal products, and foods.

Requirements

  • Student who is pursuing graduate or post graduate course in Science

Description

This 5 lecture course on HPTLC is designed to give the participant a complete understanding of the technique.

HPTLC technique, which is now official in USP has applications in various fields such as Pharmaceuticals, Herbals, Forensics, Food & Cosmetics. This technique can be used for both qualitative and quantitative analysis.

HPTLC was made official in United States Pharmacopoeia in the chapters USP 203, USP 621, and USP 1064. As HPTLC has been recognized officially by United States Pharmacopoeia, it can now be used as an analytical tool by manufacturers for the identification, fingerprinting, and quantification.

HPTLC-MS hyphenation also has an added advantage of final confirmation while testing a sample, where-in the sample identification is done using HPTLC and the confirmatory test can be carried out by the MS component.

The course starts with the basics of HPTLC and moves on to teach the method development and validation, regulatory aspects of HPTLC, Applications and the last chapter talks about HPTLC-MS and effect directed analysis. Many applications  of HPTLC are covered which will help you in better understanding.

The course can be accessed by students pursuing a degree in bioanalytical sciences, pharmaceutical sciences, herbal manufacturers, pharmaceutical manufacturers, professionals working in QA and QC field, forensic scientists, food analysts.




Who this course is for:

  • The course is for those who are pursuing pharmacy, basic sciences, chemistry, nutraceuticals

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