
Isolate and purify the target DNA, digest with restriction enzymes to create cohesive ends, ligate into a plasmid, transform bacteria, form a DNA library, and identify the desired clone.
Explore gene vectors and cloning vectors such as plasmids, phage lambda, cosmids, shuttle vectors, and yeast or bacterial artificial chromosomes, with origins, restriction sites, and selectable markers.
Develop genomic libraries by digesting genomes at restriction sites, cloning overlapping fragments into plasmid vectors in E. coli, followed by screening to identify positive clones.
Use PCR to amplify DNA segments from a small sample, employing Taq polymerase, primers, and deoxyribonucleotides through cycles in a thermal cycler, identifying microorganisms and linking crime DNA.
Explore microarray technology to study gene interactions and regulatory networks, using fluorescently labeled cDNA on glass slides, hybridization, scanning, and clustering to reveal gene expression patterns.
Compare viral vectors—retroviruses, adenoviruses, AAV, and HSV—with non-viral liposomes and direct injection methods for receptor-mediated endocytosis and internalization into target cells.
First, you will master the Polymerase Chain Reaction (PCR) — the technique that amplifies DNA for cloning, diagnosis, pathogen screening, and forensic analysis. You will learn how thermal cyclers work and why PCR is essential in every modern lab.
Next, you will explore DNA sequencing using the classic Sanger dideoxy method and automated dye-terminator sequencing. You will understand gel electrophoresis and how to read a DNA sequence.
You will then study recombinant DNA technology, including restriction enzymes, gene vectors, cloning vectors, and genomic libraries. These tools allow scientists to cut, paste, and modify genes.
The course also covers microarray and gene chip technology, with real examples in melanoma and breast carcinoma diagnosis. You will learn how gene therapy works, which diseases can be treated, and what vectors are used.
We also explore transgenic animal technology and how genetically modified organisms are created for research and agriculture.
New section added: Introduction to AI in biotechnology — including free tools for DNA analysis and research.
Who is this course for?
Medical students, veterinary students, biology students, lab technicians, researchers, and anyone curious about genetic engineering and molecular biology.
By the end of this course
You will understand PCR, DNA sequencing, cloning, microarrays, gene therapy, and how AI is shaping the future of biotechnology.
Enroll today and start your biotech journey