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UcanGE: genetic engineering
Rating: 4.3 out of 5(22 ratings)
116 students

UcanGE: genetic engineering

DNA cloning and synthetic biology
Last updated 9/2021
English

What you'll learn

  • The development of genetic engineering and synthetic biology
  • DNA cloning techniques and plasmid design
  • Uses for genetic engineered systems
  • DNA modification enzymes (DNA polymerases, restriction enzymes, nucleases, phosphatases/kinases, recombinases, and more)

Course content

3 sections12 lectures1h 41m total length
  • Promo1:31
  • Class 1. From DNA to proteins7:13

    Explore how genetic information stored in dna is transcribed to rna and translated by ribosomes into proteins, highlighting the central dogma, gene structure, and evolution across life.

  • Class 2. Genetic engineering to synthetic biology6:49

    Trace the shift from genetic engineering to synthetic biology, outlining DNA as the inheritance unit, recombinant DNA and transgenic organisms, sequencing and mass spectrometry, and omics-driven design of biological systems.

  • Class 3. Genetic elements9:02

    Explore genetic elements that regulate transcription and translation, including promoters, untranslated regions, coding sequences, terminators, and CIS factors, and learn how modular parts in plasmids assemble to control gene expression.

  • Module 1 quiz

Requirements

  • Basic knowledge of DNA, RNA, and proteins

Description


Designing, building, and testing genetic designs has created the interdisciplinary field of synthetic biology that rationally designs biological systems, most often using DNA assembly. Biological systems are fascinating because they store information that is autocatalytic and self-replicating. This course is an introduction to the techniques of genetic engineering, tips for their application, and discussion of use-cases. The first module will review key concepts of molecular biology and biological system information storage, the eras of biological research and major technological breakthroughs, and how genetic elements have come to be defined and now abstracted. The second module focuses on the DNA modification techniques widely in use, the enzymes and other components, and design principals are covered. The final module looks at the applications of these techniques for genetically modified organisms. The future of distributed synthetic biology is also highlighted. Example experiments are included at the conclusion of the course to help see the new knowledge in action.


Genetic engineering has gone from a advanced laboratories to elementary classrooms as the techniques become more widely known and materials available. To build plasmids, genetic circuits, and engineered organisms a knowledge of DNA modification techniques is needed. These tools are available to a resourceful biohacker and will be familiar at the end of the course.


Who this course is for:

  • Biohackers looking to get a broad view of genetic engineering
  • Biotechnology students interested in DNA cloning
  • Biotechnology professionals needing coverage of the foundations of synthetic biology