
Explore how genetic manipulation alters dna to produce desired traits, comparing selective breeding, genetic engineering, and genome editing, with a history from early modification to modern gm crops.
Explore the gene manipulation process—from DNA isolation with restriction endonucleases and ligation to vector-based transfer and genome editing in crops, animals, and research.
Explore how genetically modified organisms are created using recombinant DNA technology and cloning, including gene isolation, vectors, promoters, selectable markers, and delivery methods like Agrobacterium, biolistics, and microinjection.
Trace the history of GMOs from selective breeding to recombinant DNA, highlighting key milestones in transgenic animals, crops, and commercial approvals.
Examine the applications and controversies of GMOs, including edible vaccines, GM crops, vaccines, and livestock modifications. Learn how these advances aim to boost production and sustainability.
Explore major controversies over GMOs, including safety, environment, regulation, biodiversity, and ethics. Analyze stakeholder roles—consumers, producers, biotech firms, regulators, NGOs, and scientists—and case-by-case testing and global policy variation.
Explore how gene therapy treats diseases by correcting genetic problems, from traditional gene transfer to CRISPR genome editing, and distinguish germline from somatic therapies.
Gene therapy alters DNA to restore protein function by delivering normal or edited genes, using viral or nonviral vectors, ex vivo or in vivo, with CRISPR and nanoparticles shaping precision.
Assess safety of gene therapy by outlining risks and how FDA, NIH, IRB, and IBC oversight guide phase I–III trials and approvals for spinal muscular atrophy and Leber congenital amaurosis.
Examine cell-based gene therapy with CAR-T cells that express a chimeric antigen receptor to target cancer cells. Discover RNA and epigenetic therapies that modify gene expression and protein production.
ABOUT DNA MANIPULATION
Genetic engineering (also called genetic modification) is a process that uses laboratory-based technologies to alter the DNA makeup of an organism. This may involve changing a single base pair (A-T or C-G), deleting a region of DNA or adding a new segment of DNA. For example, genetic engineering may involve adding a gene from one species to an organism from a different species to produce a desired trait. Used in research and industry, genetic engineering has been applied to the production of cancer therapies, brewing yeasts, genetically modified plants and livestock, and more.
Genetic engineering, also called genetic modification or genetic manipulation, is the modification and manipulation of an organism's genes using technology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA is obtained by either isolating or copying the genetic material of interest using recombinant DNA methods or by artificially synthesizing the DNA. A construct is usually created and used to insert this DNA into the host organism. The first recombinant DNA molecule was made by Paul Berg in 1972 by combining DNA from the monkey virus SV40 with the lambda virus. As well as inserting genes, the process can be used to remove, or "knock out", genes. The new DNA can be inserted randomly, or targeted to a specific part of the genome.
IMPORTANCE OF DNA MANIPULATION TECHNIQUES
Genetic modification allows changes in genes to transfer genetic material in different organisms. It is possible to introduce foreign genes into crop plants and express these in specific tissues. Transfer of genes can help to improve crop protection. Genetic modification can be done with various viral and non viral methods. Direct manipulation of DNA sequences can lead to different expression levels. Choosing the best method for gene delivery can be a difficult task, particularly to transfer genetic material in a proper way. This review highlights, basic mechanism and importance and different methods used for efficient gene delivery. Hence, understanding of intracellular machinery plays a crucial role for designing a more effective gene delivery environment.
Job Scope
Jobs directly related to your degree include:
· Academic researcher
· Biomedical scientist
· Clinical research associate
· Clinical scientist, genomics
· Clinical scientist, immunology
· Genetic counselor
· Plant breeder/geneticist
· Research scientist (life sciences)
· Research scientist (medical)
Jobs where your degree would be useful include:
· Biotechnologist
· Data scientist
· Epidemiologist
· Forensic scientist
· Medical science liaison
· Physician associate
· Scientific laboratory technician
· Science writer
· Secondary school teacher
Salary Range Provided in Genetic Engineering field:
Candidate in jobs or training period ranges from 2-2.8 Lakh per annum
For research associate, it ranges from 3.5 lakh to 5 lakh per year.
Junior scientists vary from 6-9 lakh per annum
Whereas for a senior scientist, it varies between 15-16 lakh per annum in private companies.
Not only in India but in foreign also if you are looking for a career the average annual base salary is $159,339 to $194,895 of geneticists in the USA. For example, a doctoral researcher gets a package of more than 22 lakh per annum (i.e. € 29,060/ year) whereas for postdoctoral researchers more than 50 Lakh per annum (€50,525/year). As the experience in this field increases, the salary packages offered in both private and government sector increases.
Who can enroll?
· Students pursuing their Life Science / Biotechnology / Bioscience / BSc / BTech / MSc / MTech who aspire to work in the clinical research field.
o Frequently Asked Questions:
o What is the time limit to complete the course?
Unlimited access until you decides to complete the course. But once you have given your certification test, the course ends. If you fail in the test, you can revise the course again
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Time to test all the knowledge you have gained. Take the test at the end of the course and evaluate yourself. Every Learning Course has a test at the end.
o How to get the certificate?
To avail, the certificate, take the test at the end of the course. Clearing the test (with a minimum of 50%) will earn you the certificate. The certificate can be downloading from our website. The certificate has a unique code by which anybody can verify the certificate on our website.
What Benefits are you going to get from this course?
o Lifetime validity
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o Earn an Industry recognized Certificate.
o Hands-on Projects to practice various concepts & tools, evaluated by our lead trainer.
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o Lifetime recording access
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