
Explore dna and rna fundamentals: dna's double helix and nucleotide bases, rna's role in coding and translating codons via mRNA to proteins, and how dna sequences define genes.
Discover how CRISPR uses Cas9 to identify, cut, and paste DNA for genome editing, removing faulty genes or altering traits. Apply these techniques in medicine and agriculture while considering ethics.
Explain how the coronavirus delivers its genetic material, uses spike proteins to attach to receptors, and reproduces inside the cell, leading to spread via droplets. Draw a parallel to computer viruses and antivirus as the immune system, and preview the link to genes and gene editing in the next video.
The lecture explains how a coronavirus vaccine uses a weakened virus to stimulate antibodies that prevent infection and trigger immune destruction of the virus, linking to CRISPR concepts.
Explore CRISPR systems as bacterial immune defenses, detailing CRISPR repeats, spacer DNA, Cas enzymes, and Type I–III interference mechanisms guiding gene editing with Cas9.
Demonstrates CRISPR-Cas9 gene editing in mouse embryonic stem cells, from designing CRISPR rna and delivering Cas9 with a dna repair template to repair via homologous recombination or non-homologous end joining.
Trace CRISPR history from 1987 in E. coli and palindromic repeats to its role in bacterial immunity; explore mRNA vaccines and gene editing's cancer applications.
Harness CRISPR-Cas9 to modify patients' T-cells for cancer immunotherapy, adding a synthetic receptor to target cancer cells and removing genes that hinder receptor function and killing ability.
Compare CRISPR-Cas9 with zinc finger nucleases and TALENs, and learn why CRISPR’s single guide RNA enables fast, easy-to-use DNA editing.
Evaluate the advantages and ethics of CRISPR gene editing, including disease relief, resilient crops, and nutrient-rich foods, while weighing safety, off-target risks, and accessibility, and note recent advances in delivery.
This is a complete Bootcamp course on emerging and intriguing technology - Genome Editing using CRISPR Cas9. It will let you edit the basic component of all life on the planet, i.e, genes. Through gene editing, you can edit your characteristics such as eye color hair color, and height. It has the capability to cure major diseases like cancer and more. This course introduces you to all the basics and dives deep into the working, functionality, applications, advantages, disadvantages, advances, and limitations of the current gene-editing technology. You will learn everything from basics about cells, DNA, RNA, genes, and more. Later dive deep into CRISPR technology and gene editing. Then we discuss the ongoing research and applications of gene editing such as treatment for cancer.
Let's edit some genes!!!!
-----------------------
Contents of the course:
Section 1
Introduction
DNA\RNA
CRISPR
CORONA Virus
CORONA Vaccine
Section 2
CRISPR System
Gene Knocking Out
History, Immune system, mRNA Vaccines.
Section 3
Cancer Immunotherapy
CRISPR, ZFN's, TALENS.
Gene Editing Adv, Disadv, Advances, Limitations.
Gene editing is performed using enzymes, particularly nucleases that have been engineered to target a specific DNA sequence, where they introduce cuts into the DNA strands, enabling the removal of existing DNA and the insertion of replacement DNA. Key among gene-editing technologies is a molecular tool known as CRISPR-Cas9, a powerful technology discovered in 2012 by American scientist Jennifer Doudna, French scientist Emmanuelle Charpentier, and colleagues and refined by American scientist Feng Zhang and colleagues. CRISPR-Cas9 functioned with precision, allowing researchers to remove and insert DNA in the desired locations.