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Fundamentals Of Flow Cytometry - An Introductory Course
Rating: 4.5 out of 5(34 ratings)
83 students

Fundamentals Of Flow Cytometry - An Introductory Course

Flow Cytometry, Basics of Flow Cytometry for beginners, Cell Biology, Cellular Analysis, Flow Cytometer
Last updated 5/2024
English

What you'll learn

  • Definition of cytometry and Flow Cytometry
  • Functioning of Flow Cytometer
  • Basic principle of Flow Cytometry
  • Basic parameters of Flow Cytometry
  • Analysis of live and dead cells by Flow Cytometry
  • Analysis of Apoptosis by Annexin-PI Flow Cytometry
  • Analysis of Cell Cycle by Flow Cytometry
  • Immunophenotyping by Flow Cytometry
  • Analysis of Intracellular markers by Flow Cytometry
  • Other applications of Flow Cytometry

Course content

7 sections11 lectures1h 11m total length
  • Introduction to the course3:20

    Learn the fundamentals of flow cytometry, including how a flow cytometer analyzes light from single cells and measures forward scatter, side scatter, and fluorescence for immunophenotyping and cell cycle analysis.

  • Definitions of Cytometry and Flow Cytometry1:29

    Cytometry measures physical and chemical properties of a single cell, and flow cytometry applies these measurements in a fluid stream using a flow cytometer.

  • Basic functioning of a Flow Cytometer4:19

    Understand how a flow cytometer converts samples into a single cell suspension, uses hydrodynamic focusing to align cells, and measures size and fluorescence via detectors.

  • Principle of Flow Cytometry and Basic parameters7:23

    Principle of flow cytometry analyzes one cell at a time as it interacts with a laser, yielding forward scatter for size, side scatter for granularity, and fluorescence.

  • Understanding the principle of Flow Cytometry by using a virtual experiment8:21

    Perform a virtual flow cytometry experiment on PBMCs, labeling T cells with CD3 and B cells with CD19, then analyze FSC, SSC, and fluorescence to identify lymphocytes, monocytes, and granulocytes.

Requirements

  • Basic knowledge about cells
  • Enthusiasm of learning the new techniques of cell biology and life sciences
  • Enthusiasm of learning Biology

Description

Flow cytometry is a modern and potent tool of life sciences and biological sciences. Whenever I think about this technology and its applications, I get mesmerized by the fact a single cell can be analyzed so quickly for its physical and chemical properties and this process can be repeated for millions of cells. This introductory course will help you to understand the meanings of words like cytometry immunophenotyping, hydrodynamic focusing and many more. This course is very suitable even for those students who have just begun their journey of experimental biology.

This course starts its journey with the discussions on basic principle of flowcytometry, the functioning of instrument and definitions of basic parameters like FSC, SSC and fluorescence signal. After the fundamental discussion this course takes you through various important applications of flowcytometry like live dead cell analysis, apoptosis analysis, cell cycle analysis, immunophenotyping and intracellular marker analysis etc.

The best part about this course is that to explain different applications the help of virtual experiments has been taken and original flowcytometry data and graphs have been used.

Lets Enjoy this introductory journey of flowcytometry with me and add the knowledge of this important analytical tool in your academic experience.

Who this course is for:

  • Bachelor's students of various fields of Biology and Life Sciences
  • Master's students of various fields of Biology and Life Sciences
  • Researchers and PhD students of various fields of Biology and Life Sciences