
This course provides a comprehensive overview of modern spectroscopic techniques essential for professionals working in analytical laboratories, quality control, pharmaceutical manufacturing, materials science, and research environments. This course covers the theoretical foundations and practical applications of ultraviolet-visible (UV-Vis), infrared (IR), and Raman spectroscopy—the three most widely used spectroscopic methods in industry today.
Students will explore how electromagnetic radiation interacts with matter at the atomic and molecular level, learning to interpret absorption, emission, and scattering processes through quantum mechanical energy level diagrams. The course emphasizes hands-on understanding of spectrometer design, operation, and maintenance, including dispersive and Fourier Transform instruments, with detailed coverage of sources, detectors, monochromators, and optical components.
Practical skills development includes proper sample preparation techniques for gases, liquids, and solids; calibration procedures; data acquisition and processing; spectral interpretation using correlation charts and reference libraries; and quantitative analysis using Beer-Lambert law and calibration curves. Students will learn to troubleshoot common instrumental problems, recognize and mitigate interferences such as fluorescence and stray light, and select appropriate techniques for specific analytical challenges.
Advanced topics include Attenuated Total Reflectance (ATR), Surface Enhanced Raman Spectroscopy (SERS), resonance Raman techniques, and specialized applications in pharmaceuticals, polymers, forensics, and materials characterization. Throughout the course, emphasis is placed on industry best practices, quality control protocols, safety considerations, and regulatory compliance requirements that working technicians encounter daily in analytical laboratories.