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UV Visible Spectroscopy-Principles and Instrumentation
Rating: 4.2 out of 5(35 ratings)
123 students

UV Visible Spectroscopy-Principles and Instrumentation

International Accredited Certificate Course in UV Visible Spectroscopy
Created byLakshmi Aswini
Last updated 1/2021
English

What you'll learn

  • Theory of Spectroscopy
  • Principle Instrumentation and Applications of UV Visible Spectroscopy
  • About Beer's Lambert's Law

Course content

1 section21 lectures2h 6m total length
  • Introduction to Author1:03
  • Contents of Theory of Spectroscopy4:41

    Spectroscopy measures radiation absorbed or emitted by atoms or molecules during rotational, vibrational, and electronic transitions. It links wavelength and frequency to photon energy and outlines uv to gamma types.

  • Types of Spectroscopy3:00
  • Theory Of Spectroscopy4:44
  • Different Ways for Emission of Absorbed Radiation2:23
  • Contents of UV Visible Spectroscopy1:01
  • Introduction of UV Visible Spectroscopy4:08

    Explore the principles and instrumentation of UV-visible spectroscopy, explaining absorption in the 200–800 nm range, electronic transitions, and the roles of sigma, pi, and non-bonding electrons.

  • Different Types of Transitions5:59
  • Principles of UV Visible Spectroscopy7:47
  • Instrumentation of UV Visible Spectroscopy and Source of Light6:59
  • Filters and Monochromators10:01
  • Sample Cells6:00
  • Detectors-Barrier Layers6:20
  • Photo Missive cells and Photo Tubes4:07
  • Different types of Instruments-Colorimeters6:59

    Identify three instrument types in UV-visible spectroscopy—calorimeter, colorimeter, and spectrophotometer—based on light source, filters, and detectors, and apply absorbance–concentration calibration to quantify samples.

  • Spectrocalorimeters and Spectrophotometers11:55
  • Terms to remember UV Visible Spectroscopy7:39
  • Beers-Lamberts Law11:24
  • Applications of Visible Spectroscopy8:09
  • Applications of UV Spectroscopy11:52
  • Thank You0:29

Requirements

  • Interest in Pharmacy related Subjects

Description

Theory of Spectroscopy, Excitation Relaxation Process, Principle, Instrumentation, Different Types of Instruments used in UV - Visible Spectroscopy and Applications of UV - Visible Spectroscopy, Types of ELectrons, Different Types of Electronic Transitions, Beer-Lambart's Law, Deviations from Beer-Labart's Law, Isobestic Point, Bathochromic Shift, Hypsochromic Shift, Hyper Chromic Effect, Hypo Chromic Effect, Chromogenic Agent.

Who this course is for:

  • Biotechnology
  • Agricultural BSc
  • Microbiology
  • PharmaD
  • B Pharmarcy
  • M Pharmacy