
Welcome to the practical world of CRISPR/Cas9 gene editing.
I am Faheem Kanwal — a student, a learner, and today, your teacher. But I didn’t start from any big lab or famous university. I started from a small lab — like many of you — with nothing in my hands except a curious heart and a dream to perform "CRISPR one day."
I still remember my first days. My PCR failed again and again. My gels were blurry. My primers didn’t work. There were days I thought — maybe this is only for big scientists in big labs. But I didn’t stop. I stayed late in my small lab, I repeated, I failed, I learned, and I dreamed even bigger.
And slowly... life started changing.
That small lab gave me my first break — I was selected for a research program at Shanghai Jiao Tong University, China, where I worked in real gene editing projects. And from there, the dream only got bigger. Today, I feel incredibly proud and blessed to share that I am going to Tsinghua University — one of the top universities in the world — to continue my journey in application of gene editing to develop future cancer therapeutics.
But this course is not about me. This course is about you.
This is about every student, every beginner, every dreamer sitting in a small lab, in any corner of the world — wondering “Can I do CRISPR too?”
My answer is YES.
In this course, I am going to teach you CRISPR/Cas9 gene editing exactly how I learned — simple, step-by-step, practical — from real experiments, not just theory.
You don’t need a big lab.
You don’t need advanced knowledge.
If you have a small lab, a learning heart, and the courage to try — that is enough to start this journey.
By the end of this course, I don’t want you to just say “I know CRISPR.”
I want you to proudly say — “I performed CRISPR with my own hands.”
Let’s begin this journey — from student to scientist.
Retrieve and visualize a gene sequence with Snap gene, exploring alternate splicing and gene maps while locating Cd90 in zebrafish via ensembl and import steps.
Explore how the bacterial CRISPR-Cas9 system becomes a powerful gene editing tool, detailing Jennifer Doudna's discovery moment, guide RNA and tracer RNA, Cas9, and DNA repair-induced edits.
A step-by-step practical journey to design primers, perform PCR, run gels, design gRNAs, do Microinjection, validate the CRISPR/Cas9 edits and carry out CRISPR gene editing — even if you’ve never worked in a lab before.
"This is This is not a typical biology theory course filled with boring information and memorization.This is a hands-on lab experience where YOU perform CRISPR/Cas9 with your own hands"
Welcome to CRISPR Practical for Beginners.
What will you learn?
→ Primer Designing — the first tool of every gene editor
→ PCR — the heartbeat of molecular biology
→ Gel Electrophoresis — watch your DNA become visible
→ Principle of Gene Editing — the story of gene editing, CRISPR/Cas9
→ gRNA Designing — create your CRISPR guide
→ Microinjection — how to inject CRISPR/Cas9 to frist cell or zygote
→ CRISPR/Cas9 Gene Editing — perform real genome editing
→ Mutation Screening — detect your edits like a scientist
Why take this course?
Because skills last forever. Money comes back. Time comes back. But the confidence to say “I performed CRISPR myself” can change your career.
Who is this for?
Every student. Every beginner. Every dreamer who once thought: “I wish I could perform CRISPR too…” Yes, you can.
Requirements
No lab experience.
No biology background.
Just curiosity.
Who am I?
I am Faheem Kanwal — once a student with shaky hands and blurry gels. From a small lab, my journey led me to Shanghai Jiao Tong University and now to Tsinghua University China. where I use CRISPR to develop future cancer therapeutics. I learned step by step, mistake by mistake — and now I’m here to teach you everything in the simplest, most practical way possible.
My Promise to You
"By the end of this course, I don’t want you to just say: “I know CRISPR.” I want you to proudly say: “I performed CRISPR with my own hands.”