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Comprehensive Medical Microbiology Course
Rating: 4.5 out of 5(167 ratings)
662 students

Comprehensive Medical Microbiology Course

Learn the diagnostic procedures of the Medical Microbiology laboratory and prepare for international certifications.
Created byYasser Alkeshan
Last updated 11/2021
English

What you'll learn

  • Intensive course for all microbiology sub-sections, including Bacteriology, Mycobacteriology, Mycology, Virology, and Parasitology.
  • Identify the pathogenic organisms and normal flora in the different types of bacteriology culture.
  • Perfect photos for the colony morphology, pigmentation, and gram stain of more than 50 types of pathogenic bacteria.
  • Interpret the bacterial growth using comprehensive flowcharts and the guidelines.
  • The basic and advanced methods of identification and susceptibility testing.
  • Phenotypic and genotypic detection of superbugs, including CRO, PDR, ESBL, MRSA, and VRE.
  • Understand the standard culture procedures of microbiology laboratory using guidelines, scenarios, technical suggestions, and troubleshooting.
  • Culture recovery and identification of Mycobacterium tuberculosis and MOTT, colony morphology, biochemical tests, and genotypic detection.
  • Clinical and morphological classification, principles of macroscopic and microscopic examination of the fungi and moulds.
  • Cell line (viral) culture, molecular detection and classification of the viruses, including Herpesviridae, Myxoviridae, Flaviviridae, and Hepatitis viruses.
  • Screening methods guidelines to detect ova and parasites using Iron-Hematoxylin and modified Kinyoun stains.

Course content

5 sections • 41 lectures • 9h 11m total length

  • Safety Consideration - Acceptance and Rejection Criteria8:53
  • Types of Bacteriology Culture and Culture Media10:41
  • Specimen Priority2:57
  • Isolation Techniques and Types of Incubation8:16
  • Heat Fixing, Gram Stain procedure, Reading, and Troubleshooting11:48
  • Identification Procedures18:29
  • Cerebro-Spinal Fluid (CSF) and Body Fluid (BF) culture21:16

    Organisms discussed in this lecture:

    - Streptococcus agalactiae

    - Haemophilus influenzae

    - Neisseria meningitides

    - Listeria monocytogenes

    - Brucella spp

  • Tissue and H.pylori Culture13:08

    Organisms discussed in this lecture:

    - Coagulase Negative Staphylococci (CoNS)

    - Bacteroides fragilis

    - Helicobacter pylori

  • Blood Culture. A31:28

    Organisms discussed in this lecture:

    - Pseudomonas aeruginosa

    - Serratia marcescens

    - Stenotrophomonas maltophilia

  • Blood Culture. B16:22

    Organisms discussed in this lecture:

    - HACEK Group

    - Propionibacterium acnes

    - Nutritionally Variant Streptococcus

  • Respiratory Culture41:14

    Organisms discussed in this lecture:

    - Streptococcus pneumoniae

    - Acinetobacter baumannii

    - Burkholderia cepacia

    - Elizabethkingia meningoseptica

    - Satellitism Phenomenon

    - Serratia marcescens

    - Moraxella catarrhalis

    - Legionella pneumophilia

    - Bordetella

    - Mycoplasma

    - Ureaplasma

  • Ear, Eye, Oral, and Throat Culture12:03

    Organisms discussed in this lecture:

    - Candida albicans

    - Streptococcus pyogenes

    - Corynebacterium diphtheriae

    - Arcanobacterium haemolyticum

  • Wound Culture17:58

    Organisms discussed in this lecture:

    - Staphylococcus aureus

    - Methicillin Resistant Staphylococcus Aureus

    - Clostridium tetani

    - Clostridium perfringens

    - Bacillus anthracis

  • Drain Fluid and Vascular Tip Culture6:26
  • Stool Culture32:52

    Organisms discussed in this lecture:

    - Salmonella

    - Shigella

    - Vibrio cholerae

    - Aeromonas

    - Plesiomonas

    - E.coli 0157

    - Campylobacter jejuni

    - Yersinia enterocolitica

    - Clostridium difficile

    - Clostridium botulinum

  • Urine Culture23:47

    Organisms discussed in this lecture:

    - Escherichia coli

    - Klebsiella pneumoniae

    - Proteus mirabilis

    - Enterococcus faecium

    - Enterococcus faecalis

    - Staphylococcus saprophyticus

  • Genital Culture25:46

    Organisms discussed in this lecture:

    -  Gardnerella vaginalis

    - Neisseria gonorrhoeae

    - Treponeme pallidum spp

  • Identification Methods22:34
  • Antibiotic Susceptibility Testing, Classification, and CLSI17:17
  • Antibiotic Susceptibility Testing Methods19:09
  • Mechanisms of Multi-Drug Resistance and Synergy Testing25:49
  • Classification and Detection of β-lactamases18:24

    - Classification of β-lactamases (Ambler Classification).

    - Phenotypic and Genotypic Detection of β-lactamases.

  • Bacteriology Practical Test

Requirements

  • General principles of medical microbiology.

Description

This comprehensive course covers all major branches of medical microbiology, including Bacteriology, Mycobacteriology, Mycology, Virology, and Parasitology.


Bacteriology

  • Step-by-Step Laboratory Workflow: Covers the complete process, from sample collection and media preparation to streaking, Gram staining, incubation, and organism identification.

  • Pathogens vs. Normal Flora: Learn the most common pathogens for each culture type, compared to normal flora, to understand key focus points during culture reading.

  • Study of Bacteria: Examine over 50 bacteria, including common species like E. coli and Pseudomonas aeruginosa, as well as rare pathogens such as Elizabethkingia meningoseptica and the HACEK group.

  • Detection of Resistant Organisms: Focus on identifying resistant strains, including Carbapenem-Resistant Enterobacteriaceae (CRE), Methicillin-Resistant Staphylococcus aureus (MRSA), Vancomycin-Resistant Enterococcus (VRE), and other Multidrug-Resistant Organisms (MDRO).

  • CLSI Guidelines in AST Testing: Understanding the Role of the Clinical and Laboratory Standards Institute (CLSI) Guidelines in Antimicrobial Susceptibility Testing (AST).

  • Classification and Reporting of Antibiotics: Learn proper classification methods and systematic reporting of antibiotic effectiveness.

  • Antimicrobial Susceptibility Testing (AST): Overview of AST methods and interpretation of results.


Mycobacteriology

  • Tuberculosis & Hansen’s Disease: Clinical manifestations of Tuberculosis (TB) and Hansen’s disease (Leprosy).

  • Laboratory Procedures: Guidelines for sample collection and handling. Specimen preparation, decontamination techniques and Acid-fast staining.

  • Isolation of mycobacteria: Based on growth rate and pigment production:

    • Rapid non-chromogenic.

    • Slow non-photochromogenic.

    • Slow photochromogenic.

    • Slow scotochromogenic.


Mycology

  • Clinical Classification of Fungal Infections

  • Morphological Classification of Fungi: based on structure and growth characteristics.

  • Principles of Macroscopic Examination

  • Principles of Microscopic Examination


Virology

  • Cell culture techniques for virus culture.

  • Molecular diagnostic methods.

  • Classification and study of major viral families:

    • Herpesviridae

    • Myxoviridae

    • Flaviviridae

    • Hepatitis viruses


Parasitology

  • Staining methods: Iron-Hematoxylin and modified Kinyoun stains.

  • Guidelines for ova and parasite detection.

Who this course is for:

  • International certification seekers in medical microbiology, e.g. American Society for Clinical Pathology (ASCP) as technologists or specialists in microbiology.
  • Internal Medicine and Infectious Disease physicians to prepare for the microbiology residency exam, American Board of Bioanalysis (ABB), and American Board of Medical Microbiology (ABMM).
  • Infection Control practitioners, to prepare for the Certification Board of Infection Control (CBIC).
  • Medical Technologists, to increase their knowledge and skills in the microbiology laboratory.
  • Fresh graduates, to prepare for a job interview in the microbiology laboratory.

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