Comprehensive Course in Molecular Testing Laboratory

Learn how to establish a compliant molecular PCR laboratory with separate amplification and post-amplification areas, dedicated equipment, and controlled pressures to prevent contamination.

Set up a PCR laboratory according to NOM standards, prioritizing quality disposables, contamination control, and meticulous reagent management. Train personnel and verify controls to ensure accuracy and reproducibility.

Explore the stages of nucleic acid extraction, including cell disruption and chemical and mechanical methods, and learn how purification yields high-purity DNA or RNA for PCR and sequencing.

Explore solid-phase nucleic acid extraction methods, including silica matrices, glass particles, diatomaceous earth, and magnetic beads, and review portable device challenges for point-of-care diagnostics.

Interpret real-time PCR curves to identify sigmoidal shapes, baseline and threshold crossings, Cp values, and phase transitions, while considering instrument processing and potential inhibition or background noise.

Identify and implement quality control across PCR workflows, from nucleic acid extraction to amplification, using extraction controls, positive controls, and inhibition controls to prevent false positives and negatives.

Explore quality control in PCR part II, detailing positive and negative controls, contamination prevention, and metrics, then apply controls to electrophoresis, hybridization, restriction mapping, qPCR and sequencing, including corrective actions.

Explore quality control in polymerase chain reaction (pcr) for molecular testing laboratories, focusing on preventing false positives and false negatives through corrective actions, contamination control, and proficiency testing.

Explore quality control in real-time PCR, including negative and positive controls, extraction controls, and result interpretation to detect contamination and assay performance.