
Learn to perform in silico gene expression analysis, identify hub genes in a protein interaction network, screen drugs with docking and ADMET, perform enrichment analysis, and prepare for publication.
Identify study plan steps from GEO data to protein interaction networks using the string database, then screen drugs via the Drugs in Interaction Database and perform enrichment analysis.
Procuring data from gene expression omnibus, filter for Homo sapiens and expression profiling, then explore datasets and perform initial analysis steps for downstream article preparation.
Manipulate and organize gene expression data in google sheets, apply normalization, filter by p-value 0.05, and identify overexpressed genes in Huntington's and Parkinson's disease using box plots.
Import datasets, identify differentially expressed genes common to Huntington's and Parkinson's diseases, and use a data explorer to create a Venn diagram showing shared expression patterns.
Create a protein-protein interaction network with the string tool, identify the top ten hub genes using Cytoscape, and export a publication-ready network image for further drug exploration.
Learn to export a hub gene network to Cytoscape without the String App, using short tabular text output and CSV import, then apply top ten degree and hierarchical layouts.
Identify drugs linked to your genes using the drug gene interaction database (DGIdb) by uploading a top ten list and applying toxicity and other filters to select candidates.
Learn how the updated DGIdb lets you find drugs linked to a hub gene and build an FDA-approved ligand set for docking and virtual screening.
Master drawing 3D ligand structures with Chem Sketch, save as mol file, convert to PDB using OpenBabel, and prepare structures for docking and docking-related workflows.
Learn to screen drugs using DruLiTo and OSIRIS by setting grade and interaction criteria, evaluating ADMET properties, and applying Lipinski filters to assess drug-likeness and blood-brain barrier permeability.
A complete guide to the development of ideas for in-silico research to the publication of a paper - yes this is what you get
From idea development for research to writing and publishing a paper, yes that is what you get. Learn the basics of data analysis for gene expression analysis, molecular docking, visualization of the docked complex, enrichment analysis, microRNA identification production of publication-quality images, writing a research article properly, and using Zotero - a free reference management tool. In this course, you will be getting a proper idea of how can a set of genes can be used to design drugs and how using molecular docking techniques we can validate our results for the best drug. You will be getting a good idea of 'drug repurposing' also after the completion of the hands-on training session associated with this course. No prior knowledge or skills are required to attain this course and this course is deliberately kept straightforward for new learners.
In this course, you will get all the necessary skills required to write a research article on your own, guidelines for selecting a proper journal for your manuscript, and also you can extend these research ideas to wet-lab experiments as well.
ALL THE BEST:)