- Basic biotechnological concepts
- Be able to know r-DNA technology
Cloning vectors are used for transferring fragments of foreign DNA into a suitable host. They play an important role in selecting recombinants from non-recombinants. The vector contains features that allow for the convenient insertion of a DNA fragment into the vector or its removal from the vector, for example through the presence of restriction sites. The vector and the foreign DNA may be treated with a restriction enzyme that cuts the DNA, and DNA fragments thus generated contain either blunt ends or overhangs known as sticky ends and vector DNA and foreign DNA with compatible ends can then be joined together by molecular ligation. After a DNA fragment has been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use. There are many types of cloning vectors, but the most commonly used ones are genetically engineered plasmids. Cloning is generally first performed using Escherichia coli, and cloning vectors in E. coli include plasmids, bacteriophages (such as phage λ), cosmids, and bacterial artificial chromosomes (BACs). Some DNA, however, cannot be stably maintained in E. coli, for example very large DNA fragments, and other organisms such as yeast may be used. Cloning vectors in yeast include yeast artificial chromosomes. All these cloning vectors are explained well in this course along with their characteristic features.
Who this course is for:
- Biotech students, Pharmacy students
- 08:18Introduction to Cloning Vectors
- 11:26History and features of cloning vector
- 08:43Types of Cloning vector and Plasmid vector
- 09:27Bacteriophase cloning vector
- 07:35Cosmid cloning vector
- 09:02Yeast Artificial Chromosome (YAC) and Bacterial Artificial Chromosome (BAC)
- 07:27Human Artificial Chromosome (HAC) and Retroviral Vectors
Shilpa Subhash Raut is working as an Assistant Professor in K. K. Wagh College of Pharmacy, Nashik, one of the leading brands in the education field. She has a total teaching experience of 04 years. She has completed her B. Pharm and M. Pharm from MET's Institute of Pharmacy, Adgaon Nashik, with excellent academic records.