
Explore the fundamentals of chromatography as a separation technique, including types such as HPLC and UHPLC, and apply insights to pharmaceutical, biomedical, biochemical, clinical, forensic, and environmental settings.
Uncover the basic principles of chromatography and its applications, including stationary and mobile phases and separation mechanisms, with emphasis on HPLC, gas chromatography, tandem mass spectrometry, and affinity chromatography.
Compare affinity, gel chromatography (size exclusion), and ion exchange chromatography, their principles and instrumentation, and learn applications in protein purification and biomolecule separation.
Explore high performance liquid chromatography (HPLC) principles, instrumentation, and modes, including column types, mobile and stationary phases, gradient and isocratic elution, and detectors.
Explore HPLC troubleshooting techniques to diagnose issues like poor peak shape, baseline drift, retention time shifts, excess pressure, and detector problems, with practical remedies.
Explore high performance thin layer chromatography (hptlc) as a fast, high-resolution planar separation method, contrasting it with tlc and hplc instrumentation, detection, and applications.
Explore ultra high performance liquid chromatography (UPLC) as an advanced HPLC technique featuring 1.7 micrometer columns, higher pressures, faster separations, higher resolution, and detectors like UV-Vis and MS.
Explore the principle and instrumentation of gas chromatography, including injector, column, detectors, carrier gas, and data systems, and learn key parameters and applications.
Contents
This course mainly includes following Modern Chromatographic Techniques
Chromatography: Principle, apparatus, instrumentation, chromatographic parameters, factors affecting resolution and applications of the following: a) Thin Layer chromatography b) Ion exchange chromatography c) Gas chromatography d) High Performance Liquid chromatography e) Affinity chromatography
It will be splendid experience for the learners to take up the course who wish go for only Chromatographic Techniques in detail
The course is masterpiece to clear the basics in Modern Pharmaceutical Analytical Techniques and those who go for all the techniques at one go can take up the course " Modern Pharmaceutical Instrumental Analysis Techniques". This is short curtailed course for the beginners as well as advanced learners and covers the topic such as HPLC, HPTLC, GC, LC and Tandem and hyphenated Chromatographic Techniques in detail., however covered in individual lectures.
The course is exclusive creation of Ateos Foundation of Science Education and Research (AFSER) which is Non Profit Organization and holds statutory and mandatory requirements from Government of India , as well as Chartered under the Ministry of Corporate Affairs Government of India. AFSER courses on Udemy platform awards which is not individualized trainer certificates on this elegant platform for online Education. We wish the learners a very Happy Learning!!!