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Sequence analysis: Learn the use of BLAST
Rating: 3.7 out of 5(37 ratings)
1,261 students

Sequence analysis: Learn the use of BLAST

Bioinformatics Tool
Created byDr. Sadaf Ambreen
Last updated 12/2021
English

What you'll learn

  • Explain the use of BLAST program for comparing primary biological sequence information.
  • Selection of gene or protein sequences
  • Interpretation of Results
  • Compile file for alignment

Course content

1 section • 5 lectures • 42m total length

  • Introduction7:54
  • Lecture_2: How to select gene?10:51
  • Lecture_3: Sequence Alignment10:21
  • Lecture_4: BLAST and Results7:11
  • Lecture_5: Prepare fasta file for Phylogeny(Tree)6:41

    Learn to prepare a fasta file for phylogeny using blast and Mega X, perform sequence alignment, build a phylogenetic tree with maximum likelihood, and interpret topologies to study evolutionary relationships.

  • BLAST

Requirements

  • Biological sciences

Description

Course Description

BLAST has always been concerned with sequence alignment. The unit of genetic information of an organism is known as the gene.  Students will explore genome databases, genomic data, and multiple sequence alignment and phylogeny. This course is an excellent accompaniment to postgraduate courses specializing in genomics, cancer genomics, evolution, or molecular biology, and Bioinformatics. By learning the Basic Local Alignment Search Tool (BLAST) you can find regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.

Introduction

This course is for students who have not studied Bioinformatics at the university level. The basic concepts of the BLAST course will help all Biologists how to use BLAST for their fields e.g: Biochemistry, Biotechnology, Molecular Biology, Botany, Zoology.

Objectives

The objective of this course is to provide an orientation to the students about the basic concepts of Bioinformatics tools and databases.

Prerequisite(s) and Specific Course Requirements

Biology

Course Outcomes

After completing this course, participants will be able to

1. Perform DNA sequence analysis.

2. Identify the genomic features of an organism.

3. Perform Phylogenetic analysis


Who this course is for:

  • Beginner of Bioinformatics curious about BLAST and sequence analysis

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