Bioinformatics Mastery: Genome Engineering using CRISPR Cas9

Genome engineering or gene editing is a way of making specific changes to the DNA of a cell or organism in a controlled way. It is a type of genetic engineering in which DNA is inserted, deleted, modified, or replaced in the genome of a living organism.

During the genome editing process, a type of enzyme called “engineered nuclease” cuts the genome in a specific place. When this is repaired by the cell, a change or an edit is made at the sequence, leading to a change in characteristics of a cell or an organism.

There are 3 generations of nucleases for genome editing: (1) Zing Finger Nucleases (ZFNs), (2) TALENs (Transcription activator-like effector nuclease), (3) CRISPR/Cas9.

CRISPR/Cas9 is simpler, faster, cheaper, and more accurate than older genome editing methods and thus, it is most commonly used in the genome engineering process. CRISPR stands for Cluster Random Interspaced Short Palindromic Repeats. It is the DNA-targeting part of the system which consists of an RNA molecule, or ‘guide’ designed to bind to specific DNA bases through complementary base-paring. Cas9 is the CRISPR-associated protein 9 and is the nuclease part that cuts the DNA.

The CRISPR/Cas9 system was initially identified as the RNA-based adaptive immune system in bacteria and archaea. The native CRISPR system confers resistance to viruses by incorporating “short repeats” of the viral DNA into the bacterial genome.

CRISPR Cas9 technique can be used to treat many hereditary disorders by modifying the mutated genotypes to a normal phenotype. Other applications include the creation of cellular and animal models of human disease, Improvements in biotechnology and food production, the study of the functions of genes and gene regulatory elements, and many others.

This course introduces you to the world of genome engineering and CRISPR/Cas9. Throughout the course, we will cover essential theory knowledge to understand the genome engineering process and then will start with various bioinformatics tools like guide RNA design tools, off-target prediction tools and many others used during CRISPR/Cas9 mediated gene editing.

The detailed course structure includes;

1. Genome Editing

2. NHEJ Pathway

3. HDR Pathway

4. Restriction Enzymes

5. Zinc Finger Nucleases

6. TALENs

7. CRISPR/Cas9

8. Genome Engineering using CRISPR Cas9

9. Retrieval of mRNA of a Gene

10. CRISPRdirect

11. Retrieval of Exon Sequence

12. WU-CRISPR

13. CAS designer

14. GT-Scan

15. Off-Spotter

16. Guides

17. GPP sg RNA designer

18. CRISPR Cas9 gRNA design checker

19. CHOP CHOP

20. CRISPOR

This course is a unique blend of theory and practical, where you will learn basic theory and then perform practical analysis of Genome engineering and CRISPR/Cas9 concepts. We assure you that after taking this course, your perspective will be very different for Gene editing. So, sign up for the course and see how fun, exciting, and rewarding the genome engineering tools are. We hope this course will be worth your money and time.