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Biochemistry: USMLE( Genetic strategy of Therapeutic )
3 students

Biochemistry: USMLE( Genetic strategy of Therapeutic )

Recombinant DNA technology basis
Last updated 11/2024
Arabic

What you'll learn

  • Understand concept and applications of restriction endonucleases
  • Answer questions about medical applications of recombinant DNA
  • Understand concept and applications of gene therapy
  • Understand concept of RNA interferance

Course content

2 sections • 11 lectures • 2h 12m total length
  • Introduction13:21
  • 2-cloning restriction fragment DNA-the human genome project7:04
  • 3-producing restriction fragment13:26
  • 4-Cloning Restriction Fragments Using Vectors-Genomic Libraries14:00
  • 5-Producing cDNA by Reverse Transcription of mRNA14:41
  • 6-cDNA (Expression) Libraries-Comparison of Genomic and cDNA (Expression) Librar13:49
  • 7-MEDICAL APPLICATIONS OF RECOMBINANT DNA8:41
  • 8-gene therapy (1)12:51
  • 9-gene therapy (2)12:44
  • 10-interferance RNA11:44

Requirements

  • high school basic level of biology

Description

This course provides a comprehensive overview of recombinant DNA technology, its applications, and related concepts. Building on the foundation of Kaplan's USMLE Step 1 preparation materials, this 2-hour course delves into key topics such as:

  • Recombinant DNA Technology: Learn the fundamental techniques used to manipulate and engineer DNA, including cutting, pasting, and cloning DNA fragments.

  • Genomic and Expression Libraries: Understand how to create libraries of DNA fragments representing an organism's entire genome or the genes expressed in a particular tissue or cell type.

  • Gene Therapy: Explore the cutting-edge field of gene therapy, which aims to treat genetic diseases by introducing functional copies of genes into cells.

  • RNA Interference: Discover the powerful mechanism of RNA interference, a natural process that cells use to regulate gene expression, and its potential applications in medicine.

By the end of this course, you will have a solid grasp of these essential concepts and their relevance to modern biomedical research and clinical practice.

This course offers a concise introduction to recombinant DNA technology and its wide-ranging applications in medicine and research. Over the span of two hours, you'll explore foundational concepts like how recombinant DNA is created and utilized, including the construction of genomic and expression libraries—powerful tools that allow scientists to store and analyze genetic information. We’ll also cover the basics of gene therapy, which holds potential for treating genetic diseases by correcting faulty genes directly in patients. Finally, the course introduces RNA interference (RNAi), a method that can “turn off” specific genes and is used both in research and potential therapies. Using clear explanations and insights drawn from Kaplan’s USMLE Step 1 book, this course is designed to help beginners grasp these complex topics with ease.

Who this course is for:

  • medical biochemistry learner -
  • and for any one interested about recombinant DNA technology basis and application