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Become A Master in HPLC Technique - (Liquid Chromatography)
3,565 students
Become A Master in HPLC Technique - (Liquid Chromatography)
Become a Professional in HPLC From A to Z – The Only Comprehensive Chemistry HPLC Course with Practice Test
Created byDr Mohammad Abualrub
Last updated 9/2025
English
What you'll learn
- Know the principles of HPLC.
- Understand How HPLC Works.
- Learn The Chemistry of the Column in HPLC.
- Be Familiar with HPLC Components.
- Be qualified to get a suitable career in pharmaceutical field.
- Big opportunity to join any industrial field that they are using HPLC system.
- Use HPLC research in Chemistry or Pharmacy in your college and increase your publications.
- Obtain A Certificate of HPLC that qualifies you pass the interview and get the job you want.
Explore related topics
Course content
14 sections • 34 lectures • 1h 28m total length
Requirements
- Understanding The Basics of Analytical Chemistry.
- No prior experience with HPLC is required — this course is designed for beginners as well as those who want to refresh their knowledge.
- Desire to learn more about HPLC and a willingness to make the necessary effort.
- Interest in laboratory techniques and analytical methods will make learning easier and more engaging.
Description
Dear Friend,
This course is the first and only dedicated HPLC course exclusively on Udemy.
High-Performance Liquid Chromatography (HPLC) is one of the most widely used analytical tools in testing laboratories. It allows for the separation, identification, purification, and quantification of drug substances as well as many organic compounds.
HPLC is an incredibly powerful technique with applications across multiple fields: analytical chemistry, pharmaceuticals, food and nutrition, polymers, agricultural sciences, environmental testing, and research in universities and industry.
For students who complete training in HPLC, there are many exciting career opportunities, including:
R&D scientists
QA managers and practicing chemists
Researchers in chemistry, pharmacy, food and nutrition, polymer science, and agricultural sciences
Life science researchers
Professionals in governmental and regulatory bodies
Environmental science specialists
The ability to operate HPLC is now considered a fundamental skill for chemists and technicians in laboratories. Recent surveys from industry and academia highlight a strong demand for HPLC training, especially for diploma graduates and working professionals who intend to join testing labs but lack hands-on training opportunities. This course was designed to fill that gap by providing a comprehensive and practical introduction to HPLC concepts, operation, and applications.
Student Reviews
★★★★★ “I’m taking this course to complete my PhD research in Analytical Chemistry. I prepared a new organic compound and needed a method for analysis. This will increase my publication opportunities, and my supervisor is pleased. HPLC is the best method for my work.” – Forhad
★★★★★ “Finally I found what I was looking for. This course covers all the basics in HPLC. I learned so much more than in my school. Very helpful and I’m glad I took this course.” – Jonlok
★★★★★ “Outstanding experience. This course helped me increase my confidence for future job applications in the pharmaceutical industry. Now I can apply with the knowledge and certificate from this course.” – Akef Abuinsair
★★★★★ “This course covers essential information about chromatography. The explanations are step-by-step and easy to follow. Excellent course!” – Reloex
★★★★★ “A very comprehensive and well-balanced HPLC course. The instructor has excellent experience in HPLC and explains everything clearly.” – Alex
What You’ll Learn
This course will guide you through everything you need to know about HPLC and how it works. You will learn about the components of this instrument, the importance of the stationary phase (the column), and the chemistry behind separations. The content is designed for learners of all levels, regardless of prior knowledge.
I am Dr. Mohammad Abualrub, PhD in Chemistry, with more than 8 years of experience in the pharmaceutical industry and over 4 years of experience teaching chemistry. I have been using HPLC daily in laboratory settings for more than 8 years, and I bring this expertise into the course.
Course Content
Introduction to liquid chromatography
How HPLC works
HPLC analysis
Components of HPLC
Column chemistry – stationary phase
System suitability
Starting and setting up the HPLC system
Determination of assay using HPLC
Method development
You will also get the chance to see a real HPLC instrument inside an analytical chemistry lab. I will demonstrate how to install a column, prepare the mobile phase, and run the system step by step.
New! HPLC Practice Test (30 Questions)
To strengthen your learning, this course now includes a dedicated 30-question HPLC Practice Test.
Covers all major topics: theory, components, column chemistry, assay, system suitability, and method development.
Designed in multiple-choice format, just like professional exams.
Helps you evaluate your understanding, identify weak areas, and build exam-style confidence.
Estimated completion time: 40 minutes.
At the End of This Course
You will test your knowledge with quizzes and the final practice test covering essential questions such as:
What does HPLC stand for?
Typical HPLC column dimensions
Pressure range in normal analytical separations
Commonly used mobile phases in HPLC
Effect of flow rate on separation
Materials used in HPLC columns
Most commonly used HPLC detectors
Isocratic vs gradient operation
How to control retention time drift
This course is risk-free with Udemy’s 30-day money-back guarantee. If you feel you have gained no value, you can request a refund with no questions asked. I stand fully behind this training and am confident it will give you the skills you need.
Let’s start success together. Click on “Take this course” at the top right of this page and begin your journey in mastering HPLC today.
Dr. Mohammad Abualrub
PhD in Chemistry
Expert in the Pharmaceutical Industry (+8 Years)
Expert in Teaching Chemistry (+4 Years)
Who this course is for:
- Students in disciplines of Chemistry, Pharmacy, Food & Nutrition, Polymer Science, and Agricultural Sciences.
- R&D Scientists and employees.
- QA Managers & Practicing Chemists, QC employees.
- Life Science researchers and students.
- Governmental Regulatory Bodies.
- Environmental Science Students.
- Interested Students To Join the Pharmaceutical Field.