ASCP MB Molecular Biology Exam Questions Practice Test

ASCP MB (Molecular Biology) is a certification offered by the American Society for Clinical Pathology (ASCP) for laboratory professionals who specialize in molecular biology. The certification is designed to validate the knowledge and skills of individuals who work in molecular diagnostics, including the identification and analysis of genetic material and the use of molecular techniques to diagnose and monitor disease. The ASCP MB certification exam covers a range of topics related to molecular biology, including nucleic acid extraction and analysis, PCR (polymerase chain reaction), DNA sequencing, gene expression analysis, and laboratory management. The exam is designed to test the candidate's knowledge and proficiency in these areas and is offered as a computer-based test. To be eligible to take the ASCP MB exam, candidates must have a bachelor's degree from an accredited institution and either one year of full-time experience in a molecular biology laboratory or a molecular biology training program. Alternatively, candidates can also qualify for the exam with a master's or doctoral degree in a related field. Obtaining the ASCP MB certification demonstrates to employers and colleagues that an individual has a strong understanding of molecular biology principles and is proficient in the use of molecular techniques for diagnostic purposes. It can also help individuals advance their career opportunities and earn higher salaries in the field of molecular biology.

The Technologist in Molecular Biology MB(ASCP) certification examination offered by the ASCP Board of Certification (BOC) is composed of 100 questions given in a 2 hour 30 minute time frame. All exam questions are multiple-choice with one best answer. The certification exam is administered using the format of computer adaptive testing (CAT).

The MB exam questions encompass the following content areas within Molecular Biology: Molecular Science, Molecular

Techniques, Laboratory Operations, and Applications of Molecular Testing. Each of these content areas comprises a

specific percentage of the overall 100-question exam.

Introduction to the course

Provides information on

• Brief introduction on Tutor

• Benefits from the course

• Topics/Structure of the course

• Who can enroll to the course

  
  

UNIT 1 : Replication

• Central Dogma of biology

• Replication of DNA

• Semiconservative replication

• Complimentary base pairing

• Supercoil and DNA topoisomerase

• Replication fork

• DNA synthesis catalysed by DNA Polymerase

• DNA Damage and Repair

• Nucleotide Excision Repair

• Base Excision Repair

• Mismatch Repair system

  
  

UNIT 2: Transcription

• Central Dogma of Life

• what is Transcription

• Mechanism Of Transcription

• In Prokaryotes

  • Initiation

  • elongation

  • Termination

• In Eukaryotes

  • Initiation is initiated with help of promotor region

  • TAAT Box and Pribnow Box

  • Elongation

  • Enhancers and Silencers

  • Termination

• Post Transcriptional Modification

  • Addition of Cap and Poly A tail

  • Splicing Of mRNA

  • mRNA Editing

  • Inhibitors of Transcription

  • Reverse Transcriptase and RNA Degradation.

UNIT 3 : Translation

• Genetic Code

• Features Of Genetic Code

• Codon - Anticodon Pairing

• wobble Hypothesis

• Link Between Amino Acid And Nucleic Acid

  Translation In Prokaryotes

• Initiation

• Elongation

• Termination

• How much Energy is consumed during the Process

  Translation in Eukaryotes

• Initiation with Pre Initiation complex

• Association of Ribosomal Subunit

• Elongation

• Termination using Termination Codons

• Release Factors

  Post Translational Modification

• Trimming

• Structural Reorganisation

• Modification at N and C Terminal Ends

• Covalent Modification

• How much Of energy is consumed during the process of Translation

  
  

  Unit 4: Regulation of Gene Expression

• Enhancers and silencers

• House keeping and invisible genes

• One cistron -one subunit concept

• DNA organisation and Gene expression

• Covalent Modification of Histones control Gene expression

• Regulation of Gene Expression in Prokaryotes :

• Lac operon

• structure of Lac Operon

• Tryptophan regulation in Eukaryotes

• Structure of Tryptophan Operon with structural gene

Who this course is for:

• Students who are in graduation level in the field of biology, molecular biology and Biotechnology