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Antigen Antibody reactions
Rating: 4.4 out of 5(29 ratings)
1,084 students

Antigen Antibody reactions

Conceptual and fundamental learning of diagnostic tests for infectious diseases
Last updated 4/2019
English

What you'll learn

  • General Features of Antigen-Antibody reactions, Sensitivity and Specificity of a test
  • Precipitation, Flocculation, Immunodiffusion tests
  • Agglutination tests
  • Complement fixation test

Course content

4 sections4 lectures50m total length
  • Basic Concepts9:45

    Antigen antibody reactions part 1 : Introduction

    Here we will learn about

    1. Antigen

    2. Epitope

    3. Antibody

    4. Paratope

    5. General Fetures of Antigen antibody reactions

    6. Immunology

    7. Serology

    8. Affinity

    9. Avidity

    10. Sensitivity

    11. Specificity

    12. Primary stage

    13. Secondary stage

    14. Tertiary stage

Requirements

  • None. Course can be taken by any student without any prior knowledge/experience in immunology

Description

Get a conceptual and fundamental learning of diagnostic tests for infectious diseases.

Antigen-Antibody reactions

Introduction

  1. What is Antigen?

  2. What is Epitope?

  3. What is Antibody?

  4. What is Paratope ?

  5. What are the general features/characteristics of Antigen antibody reactions?

  6. What is the difference between Immunology and Serology?

  7. Understand the concept of Affinity and Avidity with examples

  8. What is Sensitivity and Specificity of a diagnostic test?

  9. What are the stages of antigen antibody reactions?

  10. Understand difference among Primary stage, Secondary stage, Tertiary stage.

Precipitation

  1. What is Precipitation?

  2. What is Flocculation ?

  3. What is Lattice hypothesis ?

  4. What are Prozone, Postzone, Zone of equivalence? What is the significance of them?

  5. Precipitation in liquid reactions with examples

  6. Ring precipitation with example

  7. Flocculation test: Slide flocculation test with example (VDRL test, RPR test) and tube flocculation with example (Kahn Test)

  8. What is Immunodiffusion ? Precipitation in gel with examples

  9. What is the principle, method and result interpretation of Oudin procedure?

  10. What is the principle, method and result interpretation of Oakley–fulthorpe procedure ?

  11. What is the principle, method and result interpretation of  Radial immunodiffusion ?

  12. What is the principle, method and result interpretation of Ouchterlony procedure ?

  13. What is the principle, method and result interpretation of Electroimmunodiffusion ?

  14. What is the principle, method and result interpretation of Counterimmunoelectrophoresis ?

  15. What is the principle, method and result interpretation of Rocket electrophoresis?

  16. What is the principle, method and result interpretation of Laurell’s electrophoresis?

Agglutination

  1. Agglutination definition, Types of agglutination reactions, Slide agglutination, Tube agglutination, heterophile agglutination, Coombs test, Direct coombs test, Indirect coombs test, Passive agglutination , Latex agglutination


    Complement Fixation test

  1. Principle

  2. Method

  3. Result interpretation

  4. Use


Who this course is for:

  • MBBS medical undergraduate students
  • Physiotherapy undergraduate students
  • BDS Dental undergraduate students
  • BSC/MSC Microbiology undergraduate and postgraduate students
  • MD Microbiology postgraduate students
  • DNB Microbiology postgraduate students
  • Optometry undergraduate students
  • MLT Medical laboratory technician course students
  • BSC nursing undergraduate students
  • Any one interested in learning microbiology/immunology/serology