Recombinant DNA technology- a game changer in cancer biology
4.0 (11 ratings)
Course Ratings are calculated from individual students’ ratings and a variety of other signals, like age of rating and reliability, to ensure that they reflect course quality fairly and accurately.
33 students enrolled

Recombinant DNA technology- a game changer in cancer biology

Anecdotes in cancer biology revealing experimental strategies that revolutionized the field of oncology
4.0 (11 ratings)
Course Ratings are calculated from individual students’ ratings and a variety of other signals, like age of rating and reliability, to ensure that they reflect course quality fairly and accurately.
33 students enrolled
Created by Sumitra Shankar
Last updated 12/2019
English
English [Auto]
Current price: $13.99 Original price: $19.99 Discount: 30% off
5 hours left at this price!
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This course includes
  • 1.5 hours on-demand video
  • 4 articles
  • 1 downloadable resource
  • Full lifetime access
  • Access on mobile and TV
  • Certificate of Completion
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What you'll learn
  • How different theories about cancer namely- chemical, chromosomal and viral, formed significant pieces of a puzzle that led to the emergence of two main concepts- Onocogenes and Tumor suppressors
  • Students will focus on how tumor viruses revealed a bigger picture of cancer and also how identification of some of the viruses contributed significant tools to the field of recombinant DNA technology
  • How cloning strategies accelerated the understanding of molecular dimension of carcinogenesis
Requirements
  • Students must be familiar with recombinant DNA tools and techniques and concepts of cloning
  • Students must know basic concepts and terms in molecular biology such as DNA, mRNA, chromosomes, mitosis, meiosis etc.
Description

This course focuses on historical theories and  cloning strategies put forth to comprehend carcinogenesis. The objective is to travel back in time to the period when all the pieces of puzzle such as chemical carcinogens, retroviruses and chromosomal mutations came together to reveal the grand scheme of cancer and propose the underlying criteria for cancer development. The highlights of this lecture series are the experimental methods developed during 1960s to 1990s which built the foundation for the current  model of cancer which stems from two concepts namely- oncogenes and tumor suppressor genes.

Who this course is for:
  • High school students, college freshers and research students who are inquisitive enough to dig deeper into the powerful observations, brilliant experimental strategies and different theories about cancer that shaped the field of cancer biology we know today
  • This course is not for absolute beginners who are looking for a typical in-depth college curriculum and not for experts in the field who are looking for advanced knowledge.
  • This course would aid research students in tapping into the collective historical wisdom of the pioneers and use them as a guide in further advancing this field.
Course content
Expand all 25 lectures 01:34:59
+ Chromosomal theory
2 lectures 11:09
Experiments to identify chromosomal aberrations
04:31
Experiments identifying DNA, mRNA and protein
06:38
Chromosomal theory
4 questions
+ Viral theory
1 lecture 04:39
Peyton Rous- RSV and Viral theory
04:39
Viral theory
3 questions
+ Tumor viruses and their role in Cancer research
4 lectures 14:26
RSV infection: fate of cells and influence on tumor formation
04:46
Proviral theory
06:14
Reverse transcriptase and Integrase
03:18

Quiz:

For your benefit, I have posted a practice test which you can do by clicking on the link below. You can view the results as soon as you complete the quiz

RSV experiments- Quiz
00:08
+ Hunt for proto-oncogenes
4 lectures 13:07
Varmus and Bishop experiment
06:14
Src Kinase and search for other oncogenes
04:02
leukemogenesis theory
02:47

Quiz time: Click on the link below to do the practice quiz for this section

Hunt for proto-oncogenes - quiz
00:04
+ Non-viral mehanism of tumorigenesis
4 lectures 09:58
DNA transfection experiment
05:24
Are viral and non-viral mechanisms different?
02:33
Different mechanism of mutations for oncogenes
01:54

Quiz time:

Let's take a quick quiz so that we know whether we are ready to dive into the next section

Click on the link below to do the self-assessment quiz.

Non-viral mehanism of tumorigenesis- Quiz
00:07
+ Discovery of tumor suppressor genes
6 lectures 27:24
Knudson hypothesis
02:09
Loss of heterozygosity
05:46
Identification of pRB gene
08:18
Identification of p53 gene
08:49

it's quiz time again. Let's take a quiz to check our understanding of tumor suppressors.

Tumor suppressors
00:03