
Explore how the Sherlock CRISPR-Cas13 kit detects SARS-CoV-2 using guide RNA and collateral reporter cleavage to generate fluorescence, with RT-RPA amplification enabling rapid 3–10 minute results.
Select a recovered Covid-19 donor, ensure two weeks post-recovery and a negative Covid-19 test, then screen for hepatitis B, hepatitis C, and HIV, and perform plasma donation by centrifugation.
Explain how polyclonal antibodies are produced by diverse B cell clones and used in plasma therapy to provide passive immunity against SARS-CoV2.
Monoclonal antibodies are mass-produced from a single B cell clone, targeting a single epitope on SARS-CoV-2 to block spike protein–ACE2 entry and potentially avoid polyclonal plasma drawbacks.
Identify two monoclonal antibody types from recovered patients: S1 RBD antibodies block ACE2 binding, and S2-epitope antibodies prevent fusion; combining both types may be more effective.
Generate fully human monoclonal antibodies against SARS-CoV-2 using transgenic humanized mice and hybridoma technology. Screen by ELISA to select antibodies against spike protein receptor binding domain and produce for patients.
Welcome to the Course “CRISPR-based diagnosis & Antibody therapies for COVID-19 Disease”. Since its discovery as a part of a bacterial defense mechanism, the CRISPR-Cas system has revolutionized the fields of genome editing and genetic engineering. Recently scientists all over the globe are trying to leverage the power of CRISPR to develop kits for rapid COVID-19 diagnosis.
So what is the CRISPR-Cas system and how it can be used for COVID-19 diagnosis - all of this will be discussed in detail in this course. Additionally, you will get a detailed understanding of CRISPR-based rapid and point-of-care diagnostic kits like Sherlock, DETECTR, FELUDA, etc.
In this course, We will also talk about isothermal nucleic acid amplification processes like RPA and LAMP that make them excellent candidates for developing low-cost, rapid, and point-of-care diagnosis.
Further, we will discuss how the test results of crispr based diagnostic kits can be visualized on the lateral flow strip. The colored lines on the strip, similar to pregnancy tests, indicate whether the COVID-19 test is positive or negative.
In this course, we will also be looking at some of these antibody therapies like Monoclonal Antibody Therapy, which has revolutionized the treatment for cancer and has also shown potential to become a treatment for COVID-19 disease. Additionally, this course will provide you a detailed explanation of plasma therapy, how it is being used for the treatment of COVID-19, its advantages, and its limitations.
This course is an invaluable resource for medical students, doctors, and students of disciplines like biotechnology, biology, immunology, genetics, molecular biology, cell biology, and bioinformatics. In the course, the most challenging concepts are presented in a simple and palatable format using animations and graphics.
With a 30-day return policy, there is nothing to lose for you here. If you feel the course is not worth your money, you can return it and get your money back. Though, we assure you that you will not be disappointed by your wise decision of buying this course.
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