Basics of Medical Microbiology

Learn about the history and fundamentals of Microbiology.
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  • Lectures 33
  • Length 5 hours
  • Skill Level Beginner Level
  • Languages English
  • Includes Lifetime access
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About This Course

Published 6/2013 English

Course Description

An Introduction to the study of bacteria, viruses, fungi, and protozoa. Topics include History of Microbiology, Prokaryotic and Eukaryotic cells, Classification,  Microscopy, Staining techniques, and Microbial growth and nutrition. Materials will include PowerPoint presentations, links to videos, and lecture notes. The course will take a month to complete. The course will be structured in such a way that there will be a progression from one concept to the next, although each lesson will be a stand alone. It will include laboratory aspects associated with Microbiology. Whether you are new to Microbiology, want to refresher course, or learn certain basic yet complex concepts, this course aims to deliver quality material which will make learning more engaging and Microbiology a more fascinating subject, specially as it relates to Medicine. 

What are the requirements?

  • Prerequisites: Basic Chemistry and Biology

What am I going to get from this course?

  • At the end of the course, you would know who are the founding fathers of Microbiology, parts of a light Microscope, and cell culture techniques, In this course, you will learn how to classify microorganisms, how to use the correct nomenclature, the different staining techniques and factors involved in microbial growth

What is the target audience?

  • This is an introductory Microbiology course. The course will benefit mostly Undergraduate and College level students.

What you get with this course?

Not for you? No problem.
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Certificate of completion.

Curriculum

Section 1: The History of Microbiology
02:02
An overview of the course "Basics in Medical Microbiology".
09:03
A brief history of Microbiology with the contributions of persons such as Zacharias Janssen,  Galileo Galilei, Robert Hooke and Anton Van Leeuwenhoek to Microscopy; introduction to the theory of abiogenesis; a look at the life cycle of a fly to better understand the first experiments conducted concerning abiognesis; divergent thoughts concerning vitalism (abiogenesis).
14:52
Controversy over the Theory of Spontaneous Generation with Francesco Redi and Lazzaro Spalanzani against and John Needham for Spontaneous Generation. The Debate led to a Contest in which Louis Pasteur, through a set of experiments, disproved the theory; an overview of Scientific Inquiry and conclusion.
11:43
The History of Microbiology at length- A study of the progress made in the knowledge of disease prevention, transmission and treatment, with the contribution of several health care reformers to this progress. 
09:43
At length-Classical Golden Age (1854-1914) - describes the main contributions of Louis Pasteur to Microbiology (apart from disproving Spontaneous Generation): Fermentation, Pasteurization; formulation of the Germ theory; his work on Silkworm disease, cholera, and on the process of attenuation and its use in vaccination against anthrax and rabies.
11:49
At length-Classical Golden Age (1854-1914)- describes the main contributions of Robert Koch to Microbiology: his work with Anthrax; development of pure cultures; Koch's postulates; responsible for the isolation of the tuberculosis bacillus and of the Cholera bacillus. Also describes the contributions of scientists who worked with viruses, including those of Dimitri Ivanowsky and Walter Reed.
08:53
At length-describes the main events during the Second Golden Age (1943-1970) Advancement of Microbial Genetics, providing evidence that DNA is the hereditary material of cells, Knowledge of two cell types: Prokaryotic and Eukaryotic, and Mass production of Antibiotics
09:26
At length- The Third Golden Age (1971-Present) - main topics: Biotechnology, Antibiotic Resistance, Emergence and Reemergence of Infecitous Diseases, Bioremediation, Bioterrorism, and Microbial Evolution
05:01
Conclusion of the History of Microbiology both - in a nutshell and at length.
11 questions

Whose work was published as "Micrographia" by the Royal Society of London?

A) Anton Van Leeuwenhoek

B) Zacharias Janssen

C) Robert Hooke 

D) Galileo Galilei

E) Lazzaro Spalanzani


Section 2: Characteristics of Cells and Viruses
07:55

This lecture focuses on the debate over what constitutes life throughout history, mainly in terms of chemistry, that is, whether organic or inorganic compounds make up living organisms and on the difficulty in defining life. It describes the four basic processes of life found in living organisms. It ends by reviewing the cell theory which states that "all living things are composed of cells". .

Prokaryotes -Characteristics, Taxon and External structures
18:07
16:34

In this part 2 of the study of Prokaryotes, we will cover the cytoplasmic membrane, which is the interface between the outer and inner environments of the cell, the cytoplasm, and the internal structures of the prokaryotes.

04:54

Part 1 of the study of Eukaryotes involves a description of the characteristics, classification, examples, structures, in general, of the Eukaryotes.

11:06

Part 2 of the study of Eukaryotes involves the description of the external structures, cytoplasmic membrane and cytoplasm of the Eukaryotes.

05:47

Part 3 of the study of Eukaryotes involves the description of the internal structures of the Eukaryotes, namely the membranous organelles.

06:19

The final part 4 of the study of Eukaryotes involves the description of the non-membranous organelles that are part of the internal structures of the Eukaryotes.

08:27

This last lecture, in the section of Characteristics of Cells and Viruses, describes the characteristics of viruses, that distinguish them from cells, the structure and shapes of viruses, and finally the Baltimore classification of viruses.

9 questions

Section 3: Microscopy
11:29

This first lecture in the section of Microscopy introduces the comparative sizes and covers the topics of size measurement system, size comparisons among the different organisms and size comparisons relative to the 3 modes of observation, and the most common size ranges of microbial agents.

06:48

Part 1 of the lecture in Light Microscopy covers the use, definition and parts of the light microscope. It also includes description of image formation and light pathway.

09:12

This part 2 of the lecture in Light Microscopy describes the main concepts related to microscopy, such as magnification, resolution power and index of refraction, as well as other configurations, apart from light microscopy, such as phase-contrast, dark-field, and fluorescent microscopy.

Scanning Electron Microscopy
05:57
Transmission Electron Microscopy
03:52
Microscopy
10 questions
Section 4: Staining techniques
Simple staining and Negative staining
05:43
Gram-staining
07:18
Acid-fast staining
12:11
Staining techniques
5 questions
Section 5: Classification of Microorganisms
Taxonomy
12:01
Hierarchical system and Nomenclature
06:37
Phyla in Domains Bacteria and Archea
10:18
Classification of Microorganisms
5 questions
Section 6: Microbial Growth and Nutrition
Microbial growth
04:39
Physical and Chemical Factors that affect Microbial Growth
11:46
Culture Media
13:28
08:41

Please note: This video does not start until 5:15 -5:17 timepoint.

Microbial Growth and Nutrition
4 questions
Section 7: Conclusion of the Course
Conclusion of Basics in Microbiology
Preview
08:13
Overview of Basics of Medical Microbiology
9 questions

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Instructor Biography

Sunita Seemanapalli, Biomedical Researcher and Instructor

Dr. Sunita Seemanapalli has over 10 years of experience in biomedical research, particularly in field of Microbiology. She has a Medical Degree from the Federal University of Paraiba, Brazil. In order to better understand the basic mechanism of disease processes, she pursued graduate studies in the US. She earned an M.S. from Southern University, Baton Rouge, LA. Her Master's thesis focused on the levels of p53, a tumor suppressor protein in Human Cytomegalovirus-infected cells. She earned her Ph.D. from Louisiana State University, Baton Rouge, LA. Her Ph.D. dissertation focused on the role of Outer Surface Protein C in the pathogenesis of Borrelia burgdorferi, the agent of Lyme disease. In March 2011, Dr. Seemanapalli completed one year of post-doctoral research at Texas A&M, College Station, TX, where she developed mutant and complementation strains of Borrelia burgdorferi expressing virulent genes.

Dr. Seemanapalli worked at Pennington Biomedical Research Center in the Department of Human Genomics for 5 years. She also worked at the Hansen's Disease Center for about a year in research related to multi-drug resistant Mycobacterium tuberculosis.   

In reference to her teaching experience, Dr. Seemanapalli taught General Biology Laboratory courses as a Teaching Assistant at Southern University and as an Adjunct Instructor at the Baton Rouge Community College for one and a half years.In the Fall of 2011, Dr. Seemanapalli taught Microbiology lecture and lab course at Blinn College, a Community College located in Bryan, TX.  

Dr. Seemanapalli won several awards for excellent powerpoint presentations at local, regional and national scientific meetings. She has a first author and several co-author papers published in several peer-reviewed journals, including in the Journal of Immunology. Her expertise is in the areas of Microbiology, Molecular Biology and Immunology. She is passionate about teaching Microbiology in an engaging and interest-captivating manner. 




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