This course is dealing with mammalian cloning in general, and with a special method developed by the author and his colleagues that makes the method simple, inexpensive, efficient, reliable, and applicable under compromised conditions, even - possibly - in your garage..
This is a special subject among UDEMY courses, hard to insert in any existing category, nevertheless, I think more and more similar courses will be produced and needed. Keywords for the subject include science, biotechnology, embryology, cloning, animals, agriculture, medicine, innovation.
The course consists of 8 sections and 13 lectures. The language is simple and easy to understand for laymen (once they understand my bad pronunciation due to my deafness...). First lectures prepare the audience to the main subject by stepwise introduction re. the author, the course itself, embryology, theory and traditional practice of cloning. The main subject, handmade cloning will be discussed in two long chapters with ample video illustration. Understanding of events on the video are facilitated by the previous explanation - and may also mean a kind of exam for the students. In the final lectures, the controversial situation of cloning is described. Hopefully students will understand the situation and support our efforts, or at least won't regard cloners as evil creatures any more.
Anybody who is interested in this subject (with controversial reputation, I admit) is encouraged to enroll. This is not a demanding scientific education, just an invitation to an excursion to see our world as it is. Sometimes funny, sometimes frustrating, but at least - authentic.
Who am I? Am I really qualified to talk about cloning? What were my achievements and what are my limitations?
Subjective remarks about mammalian embryology: the system we work with; the available technology; and the suggested approach
·A short overview of history of cloning, the great contributors including God and his competitors, and an explanation why was Dolly's birth such a big event
oThe first unusual step of HMC is the removal of the "eggshell" followed by bisection of the oocyte by a handheld, not-too-very-sharp blade
Other tricky steps are required to overcome the difficulties created by the handmade situation: pairing with somatic cells, fusion, and special embryo culture
§A tentative list of items including prices, human qualities and legal conditions
What is animal cloning good for? Why do we regard it extremely important? What is achievable, and what are the limits - right now?
An illustrated overview about animal species already cloned and the use of cryopreservation and transgenesis alongside with cloning
Students will learn the frustrating situation of cloning research and application worldwide. Various factors hampering the use of cloning will be discussed
In this final section the actual - desperating - situation of cloning will be summarized, and an optimistic perspective (with expected pivotal role of HMC) will be outlined. .
Gábor Vajta is Adjunct Professor at Central Queensland University, Rockhampton, Australia and Honorary Professor of BGI Shenzhen, China. He lives in Cairns, Queensland, Australia, spends half of his time in China and other Asian, European and American countries. He is director of a consulting company providing services in human and domestic animal embryology all over the world. He is author of more than 160 publications (books, book chapters and papers in peer-reviewed journals, with approx. 8,500 citations). Main topics of his research include cryopreservation of embryos and oocytes , cloning by somatic cell nuclear transfer, and various aspects of mammalian embryo culture.